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SOD2 antibody [GT1165]

Recombinant antibody Orthogonal
Anti-SOD2 antibody [GT1165] used in Western Blot (WB). GTX09013
Anti-SOD2 antibody [GT1165] used in Western Blot (WB). GTX09013
Anti-SOD2 antibody [GT1165] used in IHC (Paraffin sections) (IHC-P). GTX09013
Anti-SOD2 antibody [GT1165] used in IHC (Paraffin sections) (IHC-P). GTX09013
Anti-SOD2 antibody [GT1165] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX09013

Cat. No. GTX09013

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1165

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all SOD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from hmuan SOD2

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

superoxide dismutase 2 , IPO-B , IPOB , MNSOD , MVCD6 , Mn-SOD

Cellular Localization

Mitochondrion matrix

Background

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1. [provided by RefSeq, Apr 2016]

Database

Research Area

DATA IMAGES

Anti-SOD2 antibody [GT1165] used in Western Blot (WB). GTX09013

GTX09013 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody [GT1165] (GTX09013) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-SOD2 antibody [GT1165] used in Western Blot (WB). GTX09013

GTX09013 WB Image

WB analysis of various samples using GTX09013 SOD2 antibody [GT1165].
Dilution : 1:1000
Loading : 25 μg

Anti-SOD2 antibody [GT1165] used in IHC (Paraffin sections) (IHC-P). GTX09013

GTX09013 IHC-P Image

IHC-P analysis of hmuan esophageal tissue section using GTX09013 SOD2 antibody [GT1165].
Dlution : 1:100

Anti-SOD2 antibody [GT1165] used in IHC (Paraffin sections) (IHC-P). GTX09013

GTX09013 IHC-P Image

IHC-P analysis of rat ovary tissue section using GTX09013 SOD2 antibody [GT1165].
Dlution : 1:100

Anti-SOD2 antibody [GT1165] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX09013

GTX09013 ICC/IF Image

ICC/IF analysis of NIH3T3 cells using GTX09013 SOD2 antibody [GT1165].
Orange: Primary antibody
Blue: DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399