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SOD2 antibody [GT1433]

KO/KD
Anti-SOD2 antibody [GT1433] used in Western Blot (WB). GTX630559
Anti-SOD2 antibody [GT1433] used in Western Blot (WB). GTX630559
Anti-SOD2 antibody [GT1433] used in IHC (Paraffin sections) (IHC-P). GTX630559
Anti-SOD2 antibody [GT1433] used in Western Blot (WB). GTX630559
Anti-SOD2 antibody [GT1433] used in IHC (Paraffin sections) (IHC-P). GTX630559
Anti-SOD2 antibody [GT1433] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630559
Anti-SOD2 antibody [GT1433] used in Western Blot (WB). GTX630559

Cat. No. GTX630559

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1433

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SOD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain , rat brain

Predict Reactivity

Zebrafish, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SOD2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888224

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

superoxide dismutase 2 , IPO-B , IPOB , MNSOD , MVCD6 , Mn-SOD

Cellular Localization

Mitochondrion matrix

Background

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SOD2 antibody [GT1433] used in Western Blot (WB). GTX630559

GTX630559 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody [GT1433] (GTX630559) diluted at 1:2000.

Anti-SOD2 antibody [GT1433] used in Western Blot (WB). GTX630559

GTX630559 WB Image

SOD2 antibody [GT1433] detects SOD2 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
12 % SDS-PAGE
SOD2 antibody [GT1433] (GTX630559) dilution: 1:1000

Anti-SOD2 antibody [GT1433] used in IHC (Paraffin sections) (IHC-P). GTX630559

GTX630559 IHC-P Image

SOD2 antibody [GT1433] detects SOD2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SOD2 stained by SOD2 antibody [GT1433] (GTX630559) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SOD2 antibody [GT1433] used in Western Blot (WB). GTX630559

GTX630559 WB Image

SOD2 antibody [GT1433] detects SOD2 protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
12 % SDS-PAGE
SOD2 antibody [GT1433] (GTX630559) dilution: 1:1000

Anti-SOD2 antibody [GT1433] used in IHC (Paraffin sections) (IHC-P). GTX630559

GTX630559 IHC-P Image

SOD2 antibody [GT1433] detects SOD2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SOD2 stained by SOD2 antibody [GT1433] (GTX630559) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SOD2 antibody [GT1433] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630559

GTX630559 ICC/IF Image

SOD2 antibody [GT1433] detects SOD2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: SOD2 stained by SOD2 antibody [GT1433] (GTX630559) diluted at 1:100.
Blue: Hoechst 33342 staining.

Anti-SOD2 antibody [GT1433] used in Western Blot (WB). GTX630559

GTX630559 WB Image

SOD2 antibody [GT1433] detects SOD2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract |
D. 30 μg HepG2 whole cell lysate/extract
12 % SDS-PAGE
SOD2 antibody [GT1433] (GTX630559) dilution: 1:1000

REFERENCE

Application Reference

Ren J et al. Acta Biochim Biophys Sin (Shanghai) 2022; 54 (1) : 77-90 MEF2C ameliorates learning, memory, and molecular pathological changes in Alzheimer’s disease in vivo and in vitro.
Application : WB
Reactivity : Mouse
Tsai-Chun Lai et al. Part Fibre Toxicol 2022; 19 (1) : 25 Combined exposure to fine particulate matter and high glucose aggravates endothelial damage by increasing inflammation and mitophagy: the involvement of vitamin D.
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations