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SOD2 antibody

Anti-SOD2 antibody used in Western Blot (WB). GTX103230
Anti-SOD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103230
Anti-SOD2 antibody used in Western Blot (WB). GTX103230

Cat. No. GTX103230

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC

Reactivity

 Human, Zebrafish, Baboon
Package
100 μl ($399),
25 μl ($169)
See all SOD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SOD2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951975

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

superoxide dismutase 2 , IPO-B , IPOB , MNSOD , MVCD6 , Mn-SOD

Cellular Localization

Mitochondrion matrix

Background

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SOD2 antibody used in Western Blot (WB). GTX103230

GTX103230 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish liver
12% SDS PAGE
GTX103230 diluted at 1:1000

Anti-SOD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103230

GTX103230 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using SOD2 antibody (GTX103230) at 1:300 dilution.

Anti-SOD2 antibody used in Western Blot (WB). GTX103230

GTX103230 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SOD2 antibody (GTX103230) diluted at 1:1000.

REFERENCE

Application Reference

Sherwin JR et al. Endocrinology 2010; 151 (10) : 4982-93 the endometrial response to chorionic * is blunted in a baboon model of endometriosis.
Application : IHC
Reactivity : Baboon
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169