GeneTex
United States (US)

SOD2 antibody

GTX103230_39806_20160428_WB_19011506_372.jpg

Cat No. GTX103230

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC
Reactivity Human, Baboon
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Mouse, Rat, Zebrafish, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SOD2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Superoxide Dismutase 2,Ipo-B,Ipob,Mnsod,Mvcd6,Mn-Sod,Sod2

Cellular Localization

Mitochondrion matrix

Background

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103230_39806_20160428_WB_19011506_372.jpg

GTX103230 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SOD2 antibody (GTX103230) diluted at 1:1000.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf