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SOD2 antibody

Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116093
Anti-SOD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093

Cat No. GTX116093

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat, Alligator, Bird
Package
100μl ($319),
25μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

HeLa , HCT116 , mouse brain , rat brain , 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human SOD2 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Superoxide Dismutase 2,Ipo-B,Ipob,Mnsod,Mvcd6,Mn-Sod,Sod2

Cellular Localization

Mitochondrion matrix

Background

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

SOD2 antibody detects SOD2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (GTX116093) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

SOD2 antibody detects SOD2 protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (GTX116093) diluted by 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116093

GTX116093 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SOD2/ Mn SOD(GTX116093) antibody at 1:200 dilution.

Anti-SOD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX116093

GTX116093 IHC-P Image

SOD2 antibody detects SOD2 protein at mitochondria in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SOD2 antibody (GTX116093) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

SOD2 antibody detects SOD2 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (GTX116093) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (GTX116093) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE
REVIEW

SOD2 antibody Cat No. GTX116093

Rating ( Average 4.7 based on 3 users reviews)
Application
Date : Anonymous submitted on 20-Oct-2017
Score :
Application Tested : WB
Sample Species : Ms
Amount used : 30 μg
Blocking : 6% Skim milk in TBS, 4°C, 1Hr
Primary Antibody : 1:4000, 4°C, overnightHr
Lane Description : Lane 1: 3T3-L1 cells, Lane 2: Mouse Adipocyte lysate
Date : Anonymous submitted on 20-Oct-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 10 μg
Blocking : 6% Skim milk in TBS, 4°C, 1Hr
Primary Antibody : 1:4000, 4°C, overnightHr
Date : Anonymous submitted on 04-Nov-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 30μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169