GeneTex
  • Country / Location Selection

United States (US)

SOD2 antibody

Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093
Anti-SOD2 antibody used in Western Blot (WB). GTX116093

Cat. No. GTX116093

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat, Alligator, Bird
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human SOD2 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624558

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

superoxide dismutase 2 , IPO-B , IPOB , MNSOD , MVCD6 , Mn-SOD

Cellular Localization

Mitochondrion matrix

Background

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

SOD2 antibody detects SOD2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (GTX116093) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

SOD2 antibody detects SOD2 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (GTX116093) diluted by 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX116093

GTX116093 IHC-P Image

SOD2 antibody detects SOD2 protein at mitochondria in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SOD2 antibody (GTX116093) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

SOD2 antibody detects SOD2 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (GTX116093) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116093

GTX116093 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SOD2/ Mn SOD(GTX116093) antibody at 1:200 dilution.

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (GTX116093) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

The data was published in the journal Oxid Med Cell Longev in 2020.PMID: 32089770

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

The data was published in the journal BMC Res Notes in 2015.PMID: 26423686

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

The data was published in the journal Int J Mol Sci in 2018. PMID: 30279365

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

The data was published in the 2020 in Neural Plast. PMID: 33082778

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

The data was published in the 2020 in Neural Plast. PMID: 33082778

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

The data was published in the journal Acta Neuropathol Commun in 2014. PMID: 24383546

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

The data was published in the journal Front Physiol in 2019. PMID: 31040795

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

The data was published in the journal Heliyon in 2018. PMID: 30533548

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

The data was published in the journal Heliyon in 2018. PMID: 30533548

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

The data was published in the journal Heliyon in 2018. PMID: 30533548

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 29215072

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

The data was published in the journal Physiol Rep in 2015. PMID: 26603455

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 29215072

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 29215072

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

The data was published in the journal Reprod Biol Endocrinol in 2018. PMID: 29316934

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

The data was published in the journal Front Physiol in 2016. PMID: 27877138

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

The data was published in the journal PLoS One in 2016. PMID: 27537547

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

The data was published in the journal Nat Commun in 2017. PMID: 28878358

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

The data was published in the journal Nat Commun in 2017. PMID: 28878358

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

The data was published in the journal Reprod Biol Endocrinol in 2018. PMID: 29316934

Anti-SOD2 antibody used in Western Blot (WB). GTX116093

GTX116093 WB Image

The data was published in the journal Reprod Biol Endocrinol in 2018. PMID: 29316934

REFERENCE

REVIEW

SOD2 antibody Cat. No. GTX116093

Rating ( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB) ( Average 4.7 based on 3 users reviews)
Date : Anonymous submitted on 20-Oct-2017
Score :
Application Tested : WB
Sample Species : Ms
Amount used : 30 μg
Blocking : 6% Skim milk in TBS, 4°C, 1Hr
Primary Antibody : 1:4000, 4°C, 16Hr
Lane Description : Lane 1: 3T3-L1 cells, Lane 2: Mouse Adipocyte lysate
Date : Anonymous submitted on 20-Oct-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 10 μg
Blocking : 6% Skim milk in TBS, 4°C, 1Hr
Primary Antibody : 1:4000, 4°C, 16Hr
Date : Anonymous submitted on 04-Nov-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 30μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
Mariko Kogami et al. Oncol. Lett. 2023; 26:313
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169