GeneTex
United States (US)

SOD2 antibody

KO/KD
GTX116093_41801_WB_18121410_577.jpg
GTX116093_41801_20160616_WB_shRNA_watermark_18121410_269.jpg
GTX116093_41801_WB_R_brain_18121410_756.jpg
GTX116093_40366_IFA_18121410_382.jpg
GTX116093_40366_20151223_IHC-P_18121410_772.jpg
GTX116093_41801_WB_M_brain_18121410_893.jpg

Cat No. GTX116093

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat, Alligator, Bird
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

HeLa , HCT116 , mouse brain , rat brain , 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human SOD2 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

IPOB antibody, MNSOD antibody, MVCD6 antibody, SOD2 antibody, indophenoloxidase B antibody, Mn superoxide dismutase antibody, manganese-containing superoxide dismutase antibody, mangano-superoxide dismutase antibody, "superoxide dismutase [Mn], mitochondrial antibody", "superoxide dismutase 2, mitochondrial antibody"

Cellular Localization

Mitochondrion matrix

Background

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX116093_41801_WB_18121410_577.jpg

GTX116093 WB Image

SOD2 antibody detects SOD2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (GTX116093) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX116093_41801_20160616_WB_shRNA_watermark_18121410_269.jpg

GTX116093 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (GTX116093) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX116093_41801_WB_R_brain_18121410_756.jpg

GTX116093 WB Image

SOD2 antibody detects SOD2 protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (GTX116093) diluted by 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX116093_40366_IFA_18121410_382.jpg

GTX116093 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SOD2/ Mn SOD(GTX116093) antibody at 1:200 dilution.

GTX116093_40366_20151223_IHC-P_18121410_772.jpg

GTX116093 IHC-P Image

SOD2 antibody detects SOD2 protein at mitochondria in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SOD2 antibody (GTX116093) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX116093_41801_WB_M_brain_18121410_893.jpg

GTX116093 WB Image

SOD2 antibody detects SOD2 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (GTX116093) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

SOD2 antibody Cat No. GTX116093

Rating ( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB) ( Average 4.7 based on 3 users reviews)
Date : Anonymous submitted on 20-Oct-2017
Score :
Application Tested : WB
Sample Species : Ms
Amount used : 30 μg
Blocking : 6% Skim milk in TBS, 4°C, 1Hr
Primary Antibody : 1:4000, 4°C, overnightHr
Lane Description : Lane 1: 3T3-L1 cells, Lane 2: Mouse Adipocyte lysate
Date : Anonymous submitted on 20-Oct-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 10 μg
Blocking : 6% Skim milk in TBS, 4°C, 1Hr
Primary Antibody : 1:4000, 4°C, overnightHr
Date : Anonymous submitted on 04-Nov-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 30μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf