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SOD2 antibody

Orthogonal
Anti-SOD2 antibody used in Western Blot (WB). GTX13533
Anti-SOD2 antibody used in Immunohistochemistry (IHC). GTX13533
Anti-SOD2 antibody used in Western Blot (WB). GTX13533

Cat. No. GTX13533

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, EIA, IHC

Reactivity

 Human, Mouse, Rat, Rabbit, Sheep, Bovine, Dog, Hamster, Pig, Monkey, Xenopus laevis
Package
50 μg ($399)
See all SOD2 products

APPLICATION

Application Note

EIA: Use at an assay dependent dilution. IHC: Use at a concentration of 1 μg/ml. IP: Use at a concentration of 10 μg/ml. WB: Use at a dilution of 1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 26.6 kDa). Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

25 kDa. ( Note )

Positive Control

Rat brain tissue extract

PROPERTIES

Form

Liquid

Buffer

50% Glycerol, 50mM Sodium Phosphate pH7.2, 0.1% BSA

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length protein (Human).

Purification

Affinity purified
From polyclonal serum

Conjugation

Unconjugated

RRID

AB_371959

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

superoxide dismutase 2 , IPO-B , IPOB , MNSOD , MVCD6 , Mn-SOD

Cellular Localization

Mitochondrion matrix

Background

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1. [provided by RefSeq, Apr 2016]

Database

Research Area

DATA IMAGES

Anti-SOD2 antibody used in Western Blot (WB). GTX13533

GTX13533 WB Image

"Western blot analysis of MnSOD: Lane 1: MW Marker Lane 2: HeLa Cell Lysate Lane 3: Mouse Brain Tissue Extract Lane 4: Rat Brain Tissue Extract"

Anti-SOD2 antibody used in Immunohistochemistry (IHC). GTX13533

GTX13533 IHC Image

Immunohistochemistry analysis of human skeletal muscle tissue stained with MnSOD, pAb at 10μg/ml.

Anti-SOD2 antibody used in Western Blot (WB). GTX13533

GTX13533 WB Image

Orthogonal

The data was published in the journal Evid Based Complement Alternat Med in 2013.PMID: 23573126

REFERENCE

Application Reference

Chen YW et al. Evid Based Complement Alternat Med 2013; 2013 : 364519 Cardioprotective Effects of Quercetin in Cardiomyocyte under Ischemia/Reperfusion Injury.
Application : WB
Reactivity : Rat
Submit a Reference

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399