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SOS1 antibody

Comparison
Anti-SOS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133774
Anti-SOS1 antibody used in Western Blot (WB). GTX133774

Cat. No. GTX133774

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SOS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

152 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Product Note

Knockdown/Knockout validation was supported by references (PMID:35688418)

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SOS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887103

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SOS Ras/Rac guanine nucleotide exchange factor 1 , GF1 , GGF1 , GINGF , HGF , NS4 , SOS-1

Background

This gene encodes a protein that is a guanine nucleotide exchange factor for RAS proteins, membrane proteins that bind guanine nucleotides and participate in signal transduction pathways. GTP binding activates and GTP hydrolysis inactivates RAS proteins. The product of this gene may regulate RAS proteins by facilitating the exchange of GTP for GDP. Mutations in this gene are associated with gingival fibromatosis 1 and Noonan syndrome type 4. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SOS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133774

GTX133774 ICC/IF Image

SOS1 antibody detects SOS1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SOS1 stained by SOS1 antibody (GTX133774) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-SOS1 antibody used in Western Blot (WB). GTX133774

GTX133774 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with SOS1 antibody (GTX133774) diluted at 1:1000 and competitor's antibody (sc-256) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

Application Reference

Jiang FF et al. Virol Sin 2022; 37 (4) : 591-600 Phospho-proteomics identifies a critical role of ATF2 in pseudorabies virus replication.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169