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SOX13 antibody [N1C3]

Anti-SOX13 antibody [N1C3] used in Immunoprecipitation (IP). GTX104177
Anti-SOX13 antibody [N1C3] used in Western Blot (WB). GTX104177
Anti-SOX13 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104177

Cat. No. GTX104177

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SOX13 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

Molt-4

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SOX13. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951978

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SRY-box 13 , ICA12 , Sox-13

Cellular Localization

Nucleus

Background

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. It has also been determined to be a type-1 diabetes autoantigen, also known as islet cell antibody 12. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SOX13 antibody [N1C3] used in Immunoprecipitation (IP). GTX104177

GTX104177 IP Image

Immunoprecipitation of SOX13 protein from NT2D1 whole cell extracts using 5 μg of SOX13 antibody [N1C3] (GTX104177).
Western blot analysis was performed using SOX13 antibody [N1C3] (GTX104177) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SOX13 antibody [N1C3] used in Western Blot (WB). GTX104177

GTX104177 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX104177 diluted at 1:1000

Anti-SOX13 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104177

GTX104177 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SOX13(GTX104177) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169