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SOX17 antibody [3B10]

Overexpression
Anti-SOX17 antibody [3B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83580
Anti-SOX17 antibody [3B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83580
Anti-SOX17 antibody [3B10] used in Flow cytometry (FACS). GTX83580
Anti-SOX17 antibody [3B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83580
Anti-SOX17 antibody [3B10] used in Western Blot (WB). GTX83580
Anti-SOX17 antibody [3B10] used in Western Blot (WB). GTX83580

Cat. No. GTX83580

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3B10

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse, Dog
Package
100 μl ($519)
See all SOX17 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000
ICC/IF 1:50-1:100
FACS 1:100
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 177-414 of human SOX17 (NP_071899) produced in E.coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SRY-box 17 , VUR3

Cellular Localization

Nucleus

Background

Probable transcriptional activator in the premeiotic germ cells. It binds to the sequences 5'-AACAAT-'3 or 5'-AACAAAG-3'.

Database

Research Area

DATA IMAGES

Anti-SOX17 antibody [3B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83580

GTX83580 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with SOX17 plasmid using GTX83580 SOX17 antibody [3B10].

Anti-SOX17 antibody [3B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83580

GTX83580 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX83580 SOX17 antibody [3B10].

Anti-SOX17 antibody [3B10] used in Flow cytometry (FACS). GTX83580

GTX83580 FACS Image

FACS analysis of HEK293T cells transfected with either SOX17 plasmid(Red) or empty vector control plasmid(Blue) using GTX83580 SOX17 antibody [3B10].

Anti-SOX17 antibody [3B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83580

GTX83580 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX83580 SOX17 antibody [3B10].

Anti-SOX17 antibody [3B10] used in Western Blot (WB). GTX83580

GTX83580 WB Image

Overexpression

WB analysis of HEK293T cells transfected with SOX17 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83580 SOX17 antibody [3B10].
Loading : 5 ug per lane

Anti-SOX17 antibody [3B10] used in Western Blot (WB). GTX83580

GTX83580 WB Image

WB analysis of various cell lines using GTX83580 SOX17 antibody [3B10].
Loading : 10 ug per lane
Dilution : 1:200

REFERENCE

Application Reference

V?zquez-Dom?nguez I et al. Stem Cell Res 2022; 60 : 102689 Generation of a patient-derived induced pluripotent cell line (SCTCi016-A) carrying a homozygous variant in RPE65.
Application : FACS
Reactivity : Human
Liu J et al. Stem Cell Res 2020; 49 : 102010 Repair of acute liver damage with immune evasive hESC derived hepato-blasts.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519