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SOX2 antibody [10F10]

Anti-SOX2 antibody [10F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60381
Anti-SOX2 antibody [10F10] used in Western Blot (WB). GTX60381
Anti-SOX2 antibody [10F10] used in IHC (Paraffin sections) (IHC-P). GTX60381

Cat. No. GTX60381

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10F10

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all SOX2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human SOX2 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SRY-box 2 , ANOP3 , MCOPS3

Cellular Localization

Nucleus

Background

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SOX2 antibody [10F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60381

GTX60381 ICC/IF Image

ICC/IF analysis of NTERA-2 cells using GTX60381 SOX2 antibody [10F10].
Green : SOX2
Red: Actin filaments

Anti-SOX2 antibody [10F10] used in Western Blot (WB). GTX60381

GTX60381 WB Image

WB analysis of HEK293 (1) and SOX2(AA: 2-317)-hIgGFc transfected HEK293 (2) cell lysate using GTX60381 SOX2 antibody [10F10].

Anti-SOX2 antibody [10F10] used in IHC (Paraffin sections) (IHC-P). GTX60381

GTX60381 IHC-P Image

IHC-P analysis of lung cancer tissue (left) and esophageal cancer tissue (right) using GTX60381 SOX2 antibody [10F10].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399