Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [GT1352] (GTX627405) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [GT1352] (GTX627405) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [GT1352] (GTX627405) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

SOX2 antibody [GT1352] detects SOX2 protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: SOX2 antibody [GT1352] dilution: 1:50

SOX2 antibody [GT1352] detects SOX2 protein by western blot analysis.
A. 30 μg mouse ESC whole cell lysate/extract
10% SDS-PAGE
SOX2 antibody [GT1352] (GTX627405) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

SOX2 antibody [GT1352] detects SOX2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 mouse embryo.
Green: Brachyury stained by Brachyury antibody (GTX133714) diluted at 1:250.
Red: SOX2, a nucleus marker, stained by SOX2 antibody [GT1352] (GTX627405) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Sox2 antibodies detects Sox2 proteins on embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE18.5).
Green: GFAP antibody (GTX108711) diluted at 1:1000.
Red: Sox2 antibody [GT1352] (GTX627405) diluted at 1:250.

SOX2 antibody [GT1352] detects SOX2 protein by western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
10% SDS-PAGE
SOX2 antibody [GT1352] (GTX627405) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

SOX2 antibody [GT1352] detects SOX2 protein at nucleus on by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
SOX2 antibody [GT1352] (GTX627405) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SOX2 antibody detects SOX2 protein at nucleus by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: SOX2 protein stained by SOX2 antibody (GTX627405) diluted at 1:200.
Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.