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SOX2 antibody [GT1352]

Anti-SOX2 antibody [GT1352] used in Western Blot (WB). GTX627405
Anti-SOX2 antibody [GT1352] used in Western Blot (WB). GTX627405
Anti-SOX2 antibody [GT1352] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627405
Anti-SOX2 antibody [GT1352] used in Western Blot (WB). GTX627405
Anti-SOX2 antibody [GT1352] used in Flow cytometry (FACS). GTX627405
Anti-SOX2 antibody [GT1352] used in Western Blot (WB). GTX627405
Anti-SOX2 antibody [GT1352] used in IHC (Frozen sections) (IHC-Fr). GTX627405
Anti-SOX2 antibody [GT1352] used in IHC (Paraffin sections) (IHC-P). GTX627405
Anti-SOX2 antibody [GT1352] used in IHC (Paraffin sections) (IHC-P). GTX627405

Cat No. GTX627405

Host

Mouse

Clonality

Monoclonal

Clone Name

GT1352

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

NT2D1 , human ESC , mouse ESC

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Cat, Chicken, Pig, Japanese Medaka, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_11165499

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SRY-box 2 , ANOP3 , MCOPS3

Cellular Localization

Nucleus

Background

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SOX2 antibody [GT1352] used in Western Blot (WB). GTX627405

GTX627405 WB Image

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [GT1352] (GTX627405) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SOX2 antibody [GT1352] used in Western Blot (WB). GTX627405

GTX627405 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [GT1352] (GTX627405) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-SOX2 antibody [GT1352] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627405

GTX627405 ICC/IF Image

SOX2 antibody detects SOX2 protein at nucleus by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: SOX2 protein stained by SOX2 antibody (GTX627405) diluted at 1:200.
Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.

Anti-SOX2 antibody [GT1352] used in Western Blot (WB). GTX627405

GTX627405 WB Image

SOX2 antibody [GT1352] detects SOX2 protein by western blot analysis.
A. 30 μg mouse ESC whole cell lysate/extract
10% SDS-PAGE
SOX2 antibody [GT1352] (GTX627405) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SOX2 antibody [GT1352] used in Flow cytometry (FACS). GTX627405

GTX627405 FACS Image

SOX2 antibody [GT1352] detects SOX2 protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: SOX2 antibody [GT1352] dilution: 1:50

Anti-SOX2 antibody [GT1352] used in Western Blot (WB). GTX627405

GTX627405 WB Image

SOX2 antibody [GT1352] detects SOX2 protein by western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
10% SDS-PAGE
SOX2 antibody [GT1352] (GTX627405) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SOX2 antibody [GT1352] used in IHC (Frozen sections) (IHC-Fr). GTX627405

GTX627405 IHC-Fr Image

Sox2 antibodies detects Sox2 proteins on embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE18.5).
Green: GFAP antibody (GTX108711) diluted at 1:1000.
Red: Sox2 antibody [GT1352] (GTX627405) diluted at 1:250.

Anti-SOX2 antibody [GT1352] used in IHC (Paraffin sections) (IHC-P). GTX627405

GTX627405 IHC-P Image

SOX2 antibody [GT1352] detects SOX2 protein at nucleus on by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
SOX2 antibody [GT1352] (GTX627405) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SOX2 antibody [GT1352] used in IHC (Paraffin sections) (IHC-P). GTX627405

GTX627405 IHC-P Image

SOX2 antibody [GT1352] detects SOX2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 mouse embryo.
Green: Brachyury stained by Brachyury antibody (GTX133714) diluted at 1:250.
Red: SOX2, a nucleus marker, stained by SOX2 antibody [GT1352] (GTX627405) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

REVIEW

SOX2 antibody [GT1352] Cat No. GTX627405

Rating ( Average 4 based on 1 users reviews)
Application
Flow cytometry(FACS) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 11-Mar-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : hES cells
Blocking : 2% serum, 4°C, 0.5Hr
Fixation : 70% ethanol
Primary Antibody : 1:50, 4°C, 0.5Hr
Figure Note : Black: Isotype control, Green: SOX2 antibody
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169