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SOX2 antibody [GT1876]

KO/KD Comparison
Anti-SOX2 antibody [GT1876] used in IHC (Frozen sections) (IHC-Fr). GTX627404
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404
Anti-SOX2 antibody [GT1876] used in Flow cytometry (FACS). GTX627404
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404
Anti-SOX2 antibody [GT1876] used in IHC (Frozen sections) (IHC-Fr). GTX627404
Anti-SOX2 antibody [GT1876] used in Immunoprecipitation (IP). GTX627404
Anti-SOX2 antibody [GT1876] used in IHC (Frozen sections) (IHC-Fr). GTX627404

Cat. No. GTX627404

Reference 13
5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1876

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all SOX2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:500

Note :

IHC-Fr
The use of GTX627404 for zebrafish is based on WB testing. For IHC or IHC whole mount using zebrafish samples, we recommend GTX124477.
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

human ESC , zebrafish eye , NT2D1 , PC-3 , U87-MG , SK-N-SH

Predict Reactivity

Sheep, Bovine, Cat, Chicken, Pig, Japanese Medaka, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_11162865

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SRY-box 2 , ANOP3 , MCOPS3

Cellular Localization

Nucleus

Background

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SOX2 antibody [GT1876] used in IHC (Frozen sections) (IHC-Fr). GTX627404

GTX627404 IHC-Fr Image

SOX2 antibody [GT1876] detects SOX2 protein expression at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Red: SOX2 protein stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404

GTX627404 WB Image

Comparison

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [GT1876] (GTX627404) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-SOX2 antibody [GT1876] used in Flow cytometry (FACS). GTX627404

GTX627404 FACS Image

SOX2 antibody [GT1876] detects SOX2 protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: SOX2 antibody [GT1876] dilution: 1:50

Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404

GTX627404 WB Image

SOX2 antibody [GT1876] detects SOX2 protein by Western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
10 % SDS-PAGE
SOX2 antibody [GT1876] (GTX627404) dilution: 1:5000

Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404

GTX627404 WB Image

KO/KD

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [GT1876] (GTX627404) diluted at 1:500.

Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404

GTX627404 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: PC-3
C: U87-MG
D: SK-N-SH
10% SDS PAGE
GTX627404 diluted at 1:5000

Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404

GTX627404 WB Image

SOX2 antibody [GT1876] detects SOX2 protein by Western blot analysis.
A. 30 μg zebrafish eye lysate/extract
10 % SDS-PAGE
SOX2 antibody [GT1876] (GTX627404) dilution: 1:500

Anti-SOX2 antibody [GT1876] used in IHC (Frozen sections) (IHC-Fr). GTX627404

GTX627404 IHC-Fr Image

SOX2 antibody [GT1876] detects SOX2 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: SOX2 protein stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:250.
Red: PKC alpha protein stained by PKC alpha antibody [GT1876] (GTX130453) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-SOX2 antibody [GT1876] used in Immunoprecipitation (IP). GTX627404

GTX627404 IP Image

Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [GT1876] (GTX627404) or SOX2 antibody [N1C3] (GTX101057).
Western blot analysis was performed using SOX2 antibody [GT1876] (GTX627404) diluted at 1:500.
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-SOX2 antibody [GT1876] used in IHC (Frozen sections) (IHC-Fr). GTX627404

GTX627404 IHC-Fr Image

Sox2 antibodies detects Sox2 proteins on embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE18.5).
Green: GFAP antibody (GTX100850) diluted at 1:500.
Red: Sox2 antibody [GT1876] (GTX627404) diluted at 1:500.

REFERENCE

Application Reference

Mashiko T et al. Cells 2021; 10 (3) Selective Proliferation of Highly Functional Adipose-Derived Stem Cells in Microgravity Culture with Stirred Microspheres.
Application : ICC/IF
Reactivity : Human
Zhang L et al. Cancer Lett 2021; 500 : 87-97 A novel integrated system using patient-derived glioma cerebral organoids and xenografts for disease modeling and drug screening.
Application : IHC-P
Reactivity : Human
Lee KY et al. Cells 2020; 10 (1) Upregulation of CD109 Promotes the Epithelial-to-Mesenchymal Transition and Stemness Properties of Lung Adenocarcinomas via Activation of the Hippo-YAP Signaling.
Application : WB
Reactivity : Human
Ma L et al. J Cell Mol Med 2020; (Epub) Identification of novel susceptibility loci for non-syndromic cleft lip with or without cleft palate.
Application : WB
Reactivity : Zebrafish
Andreucci E et al. J Mol Med (Berl) 2020; 98 (10) : 1431-1446 The acidic tumor microenvironment drives a stem-like phenotype in melanoma cells.
Application : FACS
Reactivity : Human
Show More +
Submit a Reference

REVIEW

SOX2 antibody [GT1876] Cat. No. GTX627404

Rating
( Average 4.6 based on 5 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 4 based on 1 users reviews)
Flow cytometry(FACS)
( Average 4 based on 1 users reviews)
Anti-SOX2 antibody [GT1876] used in IHC (Paraffin sections) (IHC-P). GTX627404
Date : Anonymous submitted on 25-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : mice brain, hippocampus, 5~10μm slide
Blocking : 3% BSA in PBST, RT, 1Hr
Fixation : 4% formalin
Permeabilization /Antigen retrieval : 10mM Citrate Buffer, 0.05% Tween-20, pH6
Primary Antibody : 1:100, 4°C, > 12Hr
Anti-SOX2 antibody [GT1876] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627404
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Int J Biol Sci in 2019.PMID: 31595152
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016. PMID: 27344963
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016. PMID: 27344963
Anti-SOX2 antibody [GT1876] used in Flow cytometry (FACS). GTX627404
Date : Anonymous submitted on 11-Mar-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : hES cells
Blocking : 2% serum, 4°C, 0.5Hr
Fixation : 70% ethanol
Primary Antibody : 1:50, 4°C, 0.5Hr
Figure Note : Black : Isotype control, Green : SOX2 antibody
Submit a Review
Application Reference
Mashiko T et al. Cells 2021; 10 (3) Selective Proliferation of Highly Functional Adipose-Derived Stem Cells in Microgravity Culture with Stirred Microspheres.
Application : ICC/IF
Reactivity : Human
Zhang L et al. Cancer Lett 2021; 500:87-97 A novel integrated system using patient-derived glioma cerebral organoids and xenografts for disease modeling and drug screening.
Application : IHC-P
Reactivity : Human
Lee KY et al. Cells 2020; 10 (1) Upregulation of CD109 Promotes the Epithelial-to-Mesenchymal Transition and Stemness Properties of Lung Adenocarcinomas via Activation of the Hippo-YAP Signaling.
Application : WB
Reactivity : Human
Ma L et al. J Cell Mol Med 2020; (Epub) Identification of novel susceptibility loci for non-syndromic cleft lip with or without cleft palate.
Application : WB
Reactivity : Zebrafish
Andreucci E et al. J Mol Med (Berl) 2020; 98 (10):1431-1446 The acidic tumor microenvironment drives a stem-like phenotype in melanoma cells.
Application : FACS
Reactivity : Human
Aminuddin A et al. Sci Rep 2020; 10 (1):7885 Mitochondrial DNA alterations may influence the cisplatin responsiveness of oral squamous cell carcinoma.
Application : WB
Reactivity : Human
Peng L et al. Int J Biol Sci 2019; 15 (11):2340-2349 Generation of Stable Induced Pluripotent Stem-like Cells from Adult Zebra Fish Fibroblasts.
Application : ICC/IF
Reactivity : Zebrafish
Liangyue Peng et al. Int J Biol Sci 2019; 15 (11):2340-2349 Generation of Stable Induced Pluripotent Stem-like Cells from Adult Zebra Fish Fibroblasts.
Application : ICC/IF
Reactivity : Zebrafish
Lin YH et al. Endocr Relat Cancer 2018; 25 (12):967-979 Thyroid hormone negatively regulates tumorigenesis through suppression of BC200.
Application : WB
Reactivity : Human
Chuang HM et al. Int J Mol Sci 2018; 19 (10) Non-Canonical Regulation of Type I Collagen through Promoter Binding of SOX2 and Its Contribution to Ameliorating Pulmonary Fibrosis by Butylidenephthalide.
Application : WB
Reactivity : Human
Chang KY et al. Biochem Biophys Res Commun 2017; 493 (1):14-19 Stress stimuli induce cancer-stemness gene expression via Sp1 activation leading to therapeutic resistance in glioblastoma.
Application : WB
Reactivity : Human
Li HK et al. Sci Rep 2016; 6:28637 DDX3 Represses Stemness by Epigenetically Modulating Tumor-suppressive miRNAs in Hepatocellular Carcinoma.
Application : WB
Reactivity : Human
Huang TT et al. Oncotarget 2015; 6 (20):18012-26 The reciprocal regulation loop of Notch2 pathway and miR-23b in controlling gastric carcinogenesis.
Application : WB
Reactivity : Human
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