GeneTex
United States (US)

SOX2 antibody [N1C3]

KO/KD Comparison
GTX101507_42046_20150814_IHC-P_M_19011506_157.jpg
GTX101507_42592_20180119_WB_competitor_watermark_19011506_890.jpg
GTX101507_42046_20150605_WB_19011506_723.jpg
GTX101507_39827_IFA_mESC_19011506_356.jpg
GTX101507_41570_IHC_R_19011506_470.jpg
GTX101507_41871_20150414_IHC-P_M_19011506_369.jpg
GTX101507_41570_20150206_IHC_M_2_19011506_150.jpg
GTX101507_42620_20161129_IHC-P_H_19011506_441.jpg
GTX101507_42676_20161129_IHC-P_H_19011506_994.jpg
GTX101507_42592_20160825_WB_19011506_772.jpg
GTX101507_41591_20150730_WB_M_ESC_19011506_413.jpg
GTX101507_39827_IP_2_19011506_257.jpg
GTX101507_41899_20170126_WB_shRNA_watermark_19011506_735.jpg
GTX101507_42536_42886_IHC-Fr_M_19011506_822.jpg
GTX101507_42620_20161013_WB_19011506_642.jpg
GTX101507_42536_1_20160808_IHC-Fr_19011506_589.jpg
GTX101507_39827_IFA_hESC_19011506_384.jpg
GTX101507_39827_CT_FACS_19011506_959.jpg
GTX101507_43075_20180704_ICC_IF_19011506_190.jpg
GTX101507_42676_20161117_WB_19011519_994.jpg
GTX101507_42046_20151204_IP_19011506_561.jpg
GTX101507_42536_2_20160808_IHC-Fr_19011506_522.jpg
GTX101507_IHC_19011506_714.jpg

Cat No. GTX101507

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:500
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

NT2D1 , hESC , Mouse ESC

Predict Reactivity

Zebrafish, Sheep, Bovine, Cat, Chicken, Pig, Japanese Medaka, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.14mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ANOP3 antibody, MCOPS3 antibody, MGC2413 antibody, SOX2 antibody, transcription factor SOX2 antibody, SRY-related HMG-box gene 2 antibody, transcription factor SOX-2 antibody, SRY (sex determining region Y)-box 2 antibody

Cellular Localization

Nucleus

Background

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101507_42046_20150814_IHC-P_M_19011506_157.jpg

GTX101507 IHC-P Image

SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101507_42592_20180119_WB_competitor_watermark_19011506_890.jpg

GTX101507 WB Image

Comparison

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX101507_42046_20150605_WB_19011506_723.jpg

GTX101507 WB Image

SOX2 antibody detects SOX2 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GTX101507) at a dilution of 1:2500.

GTX101507_39827_IFA_mESC_19011506_356.jpg

GTX101507 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SOX2(GTX101507) antibody at 1:80 dilution.

GTX101507_41570_IHC_R_19011506_470.jpg

GTX101507 IHC-P Image

SOX2 antibody [N1C3] detects SOX2 protein at nucleus on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
SOX2 antibody [N1C3] (GTX101507) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101507_41871_20150414_IHC-P_M_19011506_369.jpg

GTX101507 IHC-P Image

SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse fetal brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fetal brain.
Green: SOX2 antibody [N1C3] (GTX101507) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101507_41570_20150206_IHC_M_2_19011506_150.jpg

GTX101507 IHC-P Image

SOX2 antibody [N1C3] detects SOX2 protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101507_42620_20161129_IHC-P_H_19011506_441.jpg

GTX101507 IHC-P Image

SOX2 antibody [N1C3] detects SOX2 protein at nucleus in human esophageal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101507_42676_20161129_IHC-P_H_19011506_994.jpg

GTX101507 IHC-P Image

SOX2 antibody [N1C3] detects SOX2 protein at nucleus in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101507_42592_20160825_WB_19011506_772.jpg

GTX101507 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:10000.

GTX101507_41591_20150730_WB_M_ESC_19011506_413.jpg

GTX101507 WB Image

SOX2 antibody detects SOX2 protein by western blot analysis. Mouse ESC lysate (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GTX101507) at a dilution of 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101507_39827_IP_2_19011506_257.jpg

GTX101507 IP Image

Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [N1C3] (GTX101507) or SOX2 antibody [GT1876] (GTX627404).
Western blot analysis was performed using SOX2 antibody [N1C3] (GTX101507).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101507_41899_20170126_WB_shRNA_watermark_19011506_735.jpg

GTX101507 WB Image

KO/KD

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101507_42536_42886_IHC-Fr_M_19011506_822.jpg

GTX101507 IHC-Fr Image

SOX2 antibody [N1C3] detects SOX2 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101507_42620_20161013_WB_19011506_642.jpg

GTX101507 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [GT1876] (GTX101507) diluted at 1:10000.

GTX101507_42536_1_20160808_IHC-Fr_19011506_589.jpg

GTX101507 IHC-Fr Image

SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 rat brain.
Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101507_39827_IFA_hESC_19011506_384.jpg

GTX101507 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using SOX2(GTX101507) antibody at 1:160 dilution.

GTX101507_39827_CT_FACS_19011506_959.jpg

GTX101507 FACS Image

Flow cytometry on human embryonic stem cells, staining with SOX2 (GTX101507)antibody at 1:100 dilution(purple) or rabbit IgG (black).

GTX101507_43075_20180704_ICC_IF_19011506_190.jpg

GTX101507 ICC/IF Image

SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SOX2 stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

GTX101507_42676_20161117_WB_19011519_994.jpg

GTX101507 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:10000.

GTX101507_42046_20151204_IP_19011506_561.jpg

GTX101507 IP Image

Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [N1C3] (GTX101507).
Western blot analysis was performed using SOX2 antibody [N1C3] (GTX101507).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101507_42536_2_20160808_IHC-Fr_19011506_522.jpg

GTX101507 IHC-Fr Image

SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101507_IHC_19011506_714.jpg

GTX101507 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SOX2(GTX101507) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

SOX2 antibody [N1C3] Cat No. GTX101507

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 18-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells, T24 cells
Amount used : 1* 10^5 cells
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Lane Description : Lane 1: A549 cells, Lane 2: T24 cells.
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169