GeneTex
United States (US)

SOX2 antibody [N1C3]

KO/KD Comparison
GTX101507_42046_20150814_IHC-P_M_19011506_157.jpg
GTX101507_42592_20180119_WB_competitor_watermark_19011506_890.jpg
GTX101507_42046_20150605_WB_19011506_723.jpg
GTX101507_39827_IFA_mESC_19011506_356.jpg
GTX101507_41570_IHC_R_19011506_470.jpg
GTX101507_41871_20150414_IHC-P_M_19011506_369.jpg
GTX101507_41570_20150206_IHC_M_2_19011506_150.jpg
GTX101507_42620_20161129_IHC-P_H_19011506_441.jpg
GTX101507_42676_20161129_IHC-P_H_19011506_994.jpg
GTX101507_42592_20160825_WB_19011506_772.jpg
GTX101507_41591_20150730_WB_M_ESC_19011506_413.jpg
GTX101507_39827_IP_2_19011506_257.jpg
GTX101507_41899_20170126_WB_shRNA_watermark_19011506_735.jpg
GTX101507_42536_42886_IHC-Fr_M_19011506_822.jpg
GTX101507_42620_20161013_WB_19011506_642.jpg
GTX101507_42536_1_20160808_IHC-Fr_19011506_589.jpg
GTX101507_39827_IFA_hESC_19011506_384.jpg
GTX101507_39827_CT_FACS_19011506_959.jpg
GTX101507_43075_20180704_ICC_IF_19011506_190.jpg
GTX101507_42536_2_20160808_IHC-Fr_19011506_522.jpg
GTX101507_IHC_19011506_714.jpg
GTX101507_42942_20170811_WB_19030801_611.jpg
GTX101507_42046_20151204_IP_19011506_561.jpg
GTX101507_42676_20161117_WB_19011519_994.jpg

Cat No. GTX101507

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:500
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

NT2D1 , hESC , Mouse ESC

Predict Reactivity

Zebrafish, Sheep, Bovine, Cat, Chicken, Pig, Japanese Medaka, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Sry-Box 2,Anop3,Mcops3,Sox2

Cellular Localization

Nucleus

Background

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101507_42046_20150814_IHC-P_M_19011506_157.jpg

GTX101507 IHC-P Image

SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101507_42592_20180119_WB_competitor_watermark_19011506_890.jpg

GTX101507 WB Image

Comparison

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX101507_42046_20150605_WB_19011506_723.jpg

GTX101507 WB Image

SOX2 antibody detects SOX2 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GTX101507) at a dilution of 1:2500.

GTX101507_39827_IFA_mESC_19011506_356.jpg

GTX101507 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SOX2(GTX101507) antibody at 1:80 dilution.

GTX101507_41570_IHC_R_19011506_470.jpg

GTX101507 IHC-P Image

SOX2 antibody [N1C3] detects SOX2 protein at nucleus on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
SOX2 antibody [N1C3] (GTX101507) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101507_41871_20150414_IHC-P_M_19011506_369.jpg

GTX101507 IHC-P Image

SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse fetal brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fetal brain.
Green: SOX2 antibody [N1C3] (GTX101507) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101507_41570_20150206_IHC_M_2_19011506_150.jpg

GTX101507 IHC-P Image

SOX2 antibody [N1C3] detects SOX2 protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101507_42620_20161129_IHC-P_H_19011506_441.jpg

GTX101507 IHC-P Image

SOX2 antibody [N1C3] detects SOX2 protein at nucleus in human esophageal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101507_42676_20161129_IHC-P_H_19011506_994.jpg

GTX101507 IHC-P Image

SOX2 antibody [N1C3] detects SOX2 protein at nucleus in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101507_42592_20160825_WB_19011506_772.jpg

GTX101507 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:10000.

GTX101507_41591_20150730_WB_M_ESC_19011506_413.jpg

GTX101507 WB Image

SOX2 antibody detects SOX2 protein by western blot analysis. Mouse ESC lysate (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GTX101507) at a dilution of 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101507_39827_IP_2_19011506_257.jpg

GTX101507 IP Image

Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [N1C3] (GTX101507) or SOX2 antibody [GT1876] (GTX627404).
Western blot analysis was performed using SOX2 antibody [N1C3] (GTX101507).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101507_41899_20170126_WB_shRNA_watermark_19011506_735.jpg

GTX101507 WB Image

KO/KD

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101507_42536_42886_IHC-Fr_M_19011506_822.jpg

GTX101507 IHC-Fr Image

SOX2 antibody [N1C3] detects SOX2 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101507_42620_20161013_WB_19011506_642.jpg

GTX101507 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [GT1876] (GTX101507) diluted at 1:10000.

GTX101507_42536_1_20160808_IHC-Fr_19011506_589.jpg

GTX101507 IHC-Fr Image

SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 rat brain.
Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101507_39827_IFA_hESC_19011506_384.jpg

GTX101507 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using SOX2(GTX101507) antibody at 1:160 dilution.

GTX101507_39827_CT_FACS_19011506_959.jpg

GTX101507 FACS Image

Flow cytometry on human embryonic stem cells, staining with SOX2 (GTX101507)antibody at 1:100 dilution(purple) or rabbit IgG (black).

GTX101507_43075_20180704_ICC_IF_19011506_190.jpg

GTX101507 ICC/IF Image

SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SOX2 stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

GTX101507_42536_2_20160808_IHC-Fr_19011506_522.jpg

GTX101507 IHC-Fr Image

SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101507_IHC_19011506_714.jpg

GTX101507 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SOX2(GTX101507) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101507_42942_20170811_WB_19030801_611.jpg

GTX101507 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101507_42046_20151204_IP_19011506_561.jpg

GTX101507 IP Image

Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [N1C3] (GTX101507).
Western blot analysis was performed using SOX2 antibody [N1C3] (GTX101507).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101507_42676_20161117_WB_19011519_994.jpg

GTX101507 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:10000.

REFERENCE
REVIEW

SOX2 antibody [N1C3] Cat No. GTX101507

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 18-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells, T24 cells
Amount used : 1* 10^5 cells
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Lane Description : Lane 1: A549 cells, Lane 2: T24 cells.
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169