PRODUCT
Summary
SOX2 antibody detects SOX2 protein, a basic helix-loop-helix transcription factor (predicted molecular weight of 34 kDa). SOX2 is an indispensable factor for regulating embryonic development and in the generation of inducible pluripotent stem (iPS) cells. It is also expressed in neural stem and progenitor cells. Dysregulation of SOX2 is associated with tumorigenesis.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:5000-1:20000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:1000 |
1:50-1:200 |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse ESC , hESC , NT2D1 , NT2D1
Predict Reactivity
Zebrafish, Sheep, Bovine, Cat, Chicken, Pig, Japanese Medaka, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.15 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2038021
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
SRY-box 2 , ANOP3 , MCOPS3
Cellular Localization
Nucleus
Background
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX101507 IHC-P Image
SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101507 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX101507 IHC-Fr Image
SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis. Sample: Frozen sectioned E13.5 rat brain. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
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GTX101507 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using SOX2(GTX101507) antibody at 1:160 dilution.
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GTX101507 FACS Image
Flow cytometry on human embryonic stem cells, staining with SOX2 (GTX101507)antibody at 1:100 dilution(purple) or rabbit IgG (black).
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GTX101507 IHC-Fr Image
SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI (GTX30920).
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GTX101507 IP Image
Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [N1C3] (GTX101507). Western blot analysis was performed using SOX2 antibody [N1C3] (GTX101507). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX101507 WB Image
SOX2 antibody detects SOX2 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GTX101507) at a dilution of 1:2500.
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GTX101507 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SOX2(GTX101507) antibody at 1:80 dilution.
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GTX101507 IHC-P Image
SOX2 antibody [N1C3] detects SOX2 protein at nucleus on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. SOX2 antibody [N1C3] (GTX101507) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101507 IHC-P Image
SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse fetal brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fetal brain. Green: SOX2 antibody [N1C3] (GTX101507) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04). Blue: Nuclear staining with Hoechst 33342.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101507 IHC-P Image
SOX2 antibody [N1C3] detects SOX2 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101507 IHC-P Image
SOX2 antibody [N1C3] detects SOX2 protein at nucleus in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101507 WB Image
SOX2 antibody detects SOX2 protein by western blot analysis. Mouse ESC lysate (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GTX101507) at a dilution of 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101507 IP Image
Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [N1C3] (GTX101507) or SOX2 antibody [GT1876] (GTX627404). Western blot analysis was performed using SOX2 antibody [N1C3] (GTX101507) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX101507 WB Image
Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101507 IHC-Fr Image
SOX2 antibody [N1C3] detects SOX2 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
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GTX101507 ICC/IF Image
SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SOX2 stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 μm.
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GTX101507 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101507 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SOX2(GTX101507) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101507 IHC-P Image
SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human esophageal carcinoma.SOX2 stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
REVIEW
SOX2 antibody [N1C3]
Cat. No. GTX101507
Rating
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( Average 4.8 based on 12 users reviews)
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Application
Western Blot(WB)
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( Average 4.7 based on 6 users reviews)
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Immunocytochemistry/ Immunofluorescence(ICC/IF)
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( Average 5 based on 3 users reviews)
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IHC (Paraffin sections)(IHC-P)
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( Average 5 based on 2 users reviews)
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IHC (Frozen sections)(IHC-Fr)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2016.
PMID: 27226076
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Elife in 2017.
PMID: 28537559
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal J Cancer in 2020.
PMID: 32201526
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Elife in 2017.
PMID: 28537559
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
IHC-P
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal Neural Plast in 2016.
PMID: 28018679
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
IHC-P
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal Neural Plast in 2016.
PMID: 28018679
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
IHC-Fr
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal Dis Model Mech in 2018.
PMID: 29208635
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
ICC/IF
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal J Cancer in 2020.
PMID: 32201526
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
ICC/IF
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2016.
PMID: 27802323
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Stem Cell Res Ther in 2013.
PMID: 24423398
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
ICC/IF
Sample Species :
Ceratotherium simum
Figure Note :
The data was peer reviewed and published in the journal Nat Commun in 2018.
PMID: 29973581
Date :
Anonymous submitted on 18-Jul-2016
Application Tested :
WB
Sample Species :
Hu
Sample :
A549 cells, T24 cells
Amount used :
1* 10^5 cells
Blocking :
5% milk, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Lane Description :
Lane 1: A549 cells, Lane 2: T24 cells.