GeneTex
United States (US)

SOX2 antibody

Anti-SOX2 antibody used in Western Blot (WB). GTX101506
Anti-SOX2 antibody used in Western Blot (WB). GTX101506
Anti-SOX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101506
Anti-SOX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101506
Anti-SOX2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX101506
Anti-SOX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101506
Anti-SOX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101506
Anti-SOX2 antibody used in Flow cytometry (FACS). GTX101506
Anti-SOX2 antibody used in Western Blot (WB). GTX101506
Anti-SOX2 antibody used in Western Blot (WB). GTX101506
Anti-SOX2 antibody used in Western Blot (WB). GTX101506
Anti-SOX2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX101506

Cat No. GTX101506

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-Fr, FACS
Reactivity Human, Mouse, Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

NT2D1 , A431 , Human ESC , GL261

Predict Reactivity

Rat, Sheep, Bovine, Cat, Chicken, Pig, Japanese Medaka, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Sry-Box 2,Anop3,Mcops3,Sox2

Cellular Localization

Nucleus

Background

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-SOX2 antibody used in Western Blot (WB). GTX101506

GTX101506 WB Image

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GTX101506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOX2 antibody used in Western Blot (WB). GTX101506

GTX101506 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody (GTX101506) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-SOX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101506

GTX101506 ICC/IF Image

SOX2 antibody detects SOX2 protein at nucleus by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SOX2 protein stained by SOX2 antibody (GTX101506) diluted at 1:200.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.

Anti-SOX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101506

GTX101506 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SOX2(GTX101506) antibody at 1:200 dilution.

Anti-SOX2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX101506

GTX101506 IHC-Fr Image

Sox2 antibodies detects Sox2 proteins on embryonic mouse brain by immunohistochemical analysis.
Sample:Frozen section of embryonic mouse brain (mE18.5).
Green: beta III Tubulin antibody [GT886] (GTX631830) diluted at 1:2000.
Red: Sox2 antibody (GTX101506) diluted at 1:250.

Anti-SOX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101506

GTX101506 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using SOX2(GTX101506) antibody at 1:100 dilution.

Anti-SOX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101506

GTX101506 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed human embryonic stem cell, using SOX2(GTX101506) antibody at 1:100 dilution.

Anti-SOX2 antibody used in Flow cytometry (FACS). GTX101506

GTX101506 FACS Image

Flow cytometry on human embryonic stem cells, staining with SOX2 (GTX101506)antibody at 1:50 dilution(green) or rabbit IgG (black).

Anti-SOX2 antibody used in Western Blot (WB). GTX101506

GTX101506 WB Image

Sample (30 µg of whole cell lysate)
A: GL261
10% SDS PAGE
GTX101506 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOX2 antibody used in Western Blot (WB). GTX101506

GTX101506 WB Image

Sample (20 µg of whole cell lysate)
A: Human ESC
10% SDS PAGE
GTX101506 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOX2 antibody used in Western Blot (WB). GTX101506

GTX101506 WB Image

Sample (20 µg of whole cell lysate )
A: A431 (GTX27909)
10% SDS PAGE
GTX101506 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOX2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX101506

GTX101506 IHC-Fr Image

Sox2 antibodies detects Sox2 proteins on embryonic mouse brain by immunohistochemical analysis.
Sample:Frozen section of embryonic mouse brain (mE18.5). Red: Sox2 antibody (GTX101506) diluted at 1:500.
Blue: DAPI

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
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$ 319
$ 169