FACS analysis of PFA-fixed MCF7 cells using GTX18149 SOX2 antibody.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

ICC/IF analysis of PFA-fixed U251 cells using GTX18149 SOX2 antibody.
Green : Primary antibody
Red : Actin
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

WB analysis of MCF7 nuclear (A) and U251 (B) cell lysate using GTX18149 SOX2 antibody.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer