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SOX3 antibody

Anti-SOX3 antibody used in IHC (Paraffin sections) (IHC-P). GTX129235
Anti-SOX3 antibody used in IHC (Paraffin sections) (IHC-P). GTX129235
Anti-SOX3 antibody used in Immunoprecipitation (IP). GTX129235
Anti-SOX3 antibody used in IHC (Paraffin sections) (IHC-P). GTX129235
Anti-SOX3 antibody used in Western Blot (WB). GTX129235
Anti-SOX3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129235

Cat No. GTX129235

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

MCF-7

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SOX3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SRY-box 3 , GHDX , MRGH , PHP , PHPX , SOXB

Cellular Localization

Nucleus

Background

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. Mutations in this gene have been associated with X-linked mental retardation with growth hormone deficiency. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SOX3 antibody used in IHC (Paraffin sections) (IHC-P). GTX129235

GTX129235 IHC-P Image

SOX3 antibody detects SOX3 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: SOX3 antibody (GTX129235) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SOX3 antibody used in IHC (Paraffin sections) (IHC-P). GTX129235

GTX129235 IHC-P Image

SOX3 antibody detects SOX3 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SOX3 antibody (GTX129235) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SOX3 antibody used in Immunoprecipitation (IP). GTX129235

GTX129235 IP Image

Immunoprecipitation of SOX3 protein from NT2D1 whole cell extracts using 5 μg of SOX3 antibody (GTX129235).
Western blot analysis was performed using SOX3 antibody (GTX129235) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SOX3 antibody used in IHC (Paraffin sections) (IHC-P). GTX129235

GTX129235 IHC-P Image

SOX3 antibody detects SOX3 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SOX3 antibody (GTX129235) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SOX3 antibody used in Western Blot (WB). GTX129235

GTX129235 WB Image

SOX3 antibody detects SOX3 protein by western blot analysis.
A. 30 μg MCF-7 whole cell lysate/extract
10 % SDS-PAGE
SOX3 antibody (GTX129235) dilution: 1:1000

Anti-SOX3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129235

GTX129235 ICC/IF Image

SOX3 antibody detects SOX3 protein at nucleus by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SOX3 protein stained by SOX3 antibody (GTX129235) diluted at 1:500.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.

REFERENCE

There are currently no references for SOX3 antibody (GTX129235). Be the first to share your publications with this product.

REVIEW

SOX3 antibody Cat No. GTX129235

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 20-Feb-2017
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Blocking : 4% donkey serum in 0.04% tween/PBS, 25°C, 1Hr
Fixation : 4 % PFA
Primary Antibody : 1:600, 25°C, 3Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169