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SOX4 antibody, N-term

Anti-SOX4 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX82513
Anti-SOX4 antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82513
Anti-SOX4 antibody, N-term used in Flow cytometry (FACS). GTX82513
Anti-SOX4 antibody, N-term used in Western Blot (WB). GTX82513

Cat. No. GTX82513

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all SOX4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:10-1:50
IHC-P 1:50-1:100
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 86-114 amino acids from the N-terminal region of human SOX4.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SRY-box 4 , EVI16

Cellular Localization

Nucleus

Background

This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SOX4 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX82513

GTX82513 IHC-P Image

IHC-P analysis of human brain tissue using GTX82513 SOX4 antibody, N-term.

Anti-SOX4 antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82513

GTX82513 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX82513 SOX4 antibody, N-term.
Green : SOX4
Red : Actin filament
Blue : cell nucleus
Fixation : 4% paraformaldehyde (PFA) (20 min)
Permeabilization : Triton X-100 (0.1%, 10 min)
Dilution : 1:25

Anti-SOX4 antibody, N-term used in Flow cytometry (FACS). GTX82513

GTX82513 FACS Image

FACS analysis of A2058 cells using GTX82513 SOX4 antibody, N-term.
Green : primary antibody
Blue : negative control

Anti-SOX4 antibody, N-term used in Western Blot (WB). GTX82513

GTX82513 WB Image

WB analysis of A2058 cell lysate (35ug/lane) using GTX82513 SOX4 antibody, N-term.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399