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SOX6 antibody

Anti-SOX6 antibody used in Western Blot (WB). GTX04085
Anti-SOX6 antibody used in IHC (Paraffin sections) (IHC-P). GTX04085
Anti-SOX6 antibody used in IHC (Paraffin sections) (IHC-P). GTX04085
Anti-SOX6 antibody used in Flow cytometry (FACS). GTX04085

Cat. No. GTX04085

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($429)
See all SOX6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.25-0.5μg/ml
IHC-P 2-5μg/ml
FACS 1-3μg/1x10⁶ cells
ELISA 0.1-0.5μg/ml
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄

Preservative

0.01mg sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence of human SOX6 (DQDTVMKAIQEARKMREQIQREQQQQ).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HSSOX6,SOX6,SOXD,SRYbox 6,SRY-box transcription factor 6

Cellular Localization

Nucleus

Background

This gene encodes a member of the D subfamily of sex determining region y-related transcription factors that are characterized by a conserved DNA-binding domain termed the high mobility group box and by their ability to bind the minor groove of DNA. The encoded protein is a transcriptional activator that is required for normal development of the central nervous system, chondrogenesis and maintenance of cardiac and skeletal muscle cells. The encoded protein interacts with other family members to cooperatively activate gene expression. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-SOX6 antibody used in Western Blot (WB). GTX04085

GTX04085 WB Image

WB analysis of various sample lysates using GTX04085 SOX6 antibody.
Lane 1 : human placenta tissue lysates
Lane 2 : CACO-2 lysates
Lane 3 : U2OS lysates
Dilution : 0.5 μg/mL
Loading : 50μg

Anti-SOX6 antibody used in IHC (Paraffin sections) (IHC-P). GTX04085

GTX04085 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX04085 SOX6 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution)
Dilution : 2μg/ml

Anti-SOX6 antibody used in IHC (Paraffin sections) (IHC-P). GTX04085

GTX04085 IHC-P Image

IHC-P analysis of rat testis tissue using GTX04085 SOX6 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution)
Dilution : 2μg/ml

Anti-SOX6 antibody used in Flow cytometry (FACS). GTX04085

GTX04085 FACS Image

FACS analysis of A549 cells using GTX04085 SOX6 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample control
Antibody amount : 1μg/1x10⁶ cells

REFERENCE

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REVIEW

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Package List Price ($)
$ 429