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SOX8 antibody

Anti-SOX8 antibody used in Western Blot (WB). GTX129949
Anti-SOX8 antibody used in IHC (Paraffin sections) (IHC-P). GTX129949
Anti-SOX8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129949
Anti-SOX8 antibody used in Western Blot (WB). GTX129949

Cat. No. GTX129949

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SOX8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

human brain

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SOX8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886135

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SRY-box 8

Cellular Localization

Nucleus

Background

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein may be involved in brain development and function. Haploinsufficiency for this protein may contribute to the mental retardation found in haemoglobin H-related mental retardation (ART-16 syndrome). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SOX8 antibody used in Western Blot (WB). GTX129949

GTX129949 WB Image

SOX8 antibody detects SOX8 protein by western blot analysis. Human tissue extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SOX8 antibody (GTX129949) diluted at 1:1000.

Anti-SOX8 antibody used in IHC (Paraffin sections) (IHC-P). GTX129949

GTX129949 IHC-P Image

SOX8 antibody detects SOX8 protein at nucleus in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SOX8 antibody (GTX129949) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SOX8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129949

GTX129949 ICC/IF Image

SOX8 antibody detects SOX8 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SOX8 protein stained by SOX8 antibody (GTX129949) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-SOX8 antibody used in Western Blot (WB). GTX129949

GTX129949 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX8 antibody (GTX129949) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Liu CF et al. Transl Res 2022; 242 : 66-78 Differential expression of members of SOX family of transcription factors in failing human hearts.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169