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SOX9 antibody [1B11]

Anti-SOX9 antibody [1B11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60536
Anti-SOX9 antibody [1B11] used in Western Blot (WB). GTX60536
Anti-SOX9 antibody [1B11] used in ELISA (ELISA). GTX60536
Anti-SOX9 antibody [1B11] used in Western Blot (WB). GTX60536

Cat. No. GTX60536

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1B11

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all SOX9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human SOX9(AA : 147-306) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SRY-box 9 , CMD1 , CMPD1 , SRA1 , SRXX2 , SRXY10

Cellular Localization

Nucleus

Background

The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SOX9 antibody [1B11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60536

GTX60536 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60536 SOX9 antibody [1B11].
Green : SOX9
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-SOX9 antibody [1B11] used in Western Blot (WB). GTX60536

GTX60536 WB Image

WB analysis of human SOX9 (AA: 147-306) recombinant protein using GTX60536 SOX9 antibody [1B11].

Anti-SOX9 antibody [1B11] used in ELISA (ELISA). GTX60536

GTX60536 ELISA Image

ELISA analysis of antigen using GTX60536 SOX9 antibody [1B11].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-SOX9 antibody [1B11] used in Western Blot (WB). GTX60536

GTX60536 WB Image

WB analysis of Lovo (1) and SW620 (2) cell lysate using GTX60536 SOX9 antibody [1B11].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399