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SOX9 antibody [GT1219]

Recombinant antibody
Anti-SOX9 antibody [GT1219] used in Western Blot (WB). GTX01545
Anti-SOX9 antibody [GT1219] used in IHC (Paraffin sections) (IHC-P). GTX01545
Anti-SOX9 antibody [GT1219] used in IHC (Paraffin sections) (IHC-P). GTX01545
Anti-SOX9 antibody [GT1219] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01545
Anti-SOX9 antibody [GT1219] used in Western Blot (WB). GTX01545
Anti-SOX9 antibody [GT1219] used in Immunoprecipitation (IP). GTX01545
Anti-SOX9 antibody [GT1219] used in IHC (Paraffin sections) (IHC-P). GTX01545

Cat. No. GTX01545

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1219

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all SOX9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human SOX9.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CMD1,CMPD1,SOX9,SRA1,SRXX2,SRXY10,SRYbox 9,SRY-box transcription factor 9

Cellular Localization

Nucleus

Background

The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SOX9 antibody [GT1219] used in Western Blot (WB). GTX01545

GTX01545 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SOX9 antibody [GT1219] (GTX01545) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 31658996

Anti-SOX9 antibody [GT1219] used in IHC (Paraffin sections) (IHC-P). GTX01545

GTX01545 IHC-P Image

IHC-P analysis of mouse testis tissue section using GTX01545 SOX9 antibody [GT1219].
Dilution : 1:100

Anti-SOX9 antibody [GT1219] used in IHC (Paraffin sections) (IHC-P). GTX01545

GTX01545 IHC-P Image

IHC-P analysis of human colon tissue section using GTX01545 SOX9 antibody [GT1219].
Dilution : 1:100

Anti-SOX9 antibody [GT1219] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01545

GTX01545 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX01545 SOX9 antibody [GT1219].
Blue : DAPI

Anti-SOX9 antibody [GT1219] used in Western Blot (WB). GTX01545

GTX01545 WB Image

WB analysis of various samples using GTX01545 SOX9 antibody [GT1219].
Dilution : 1:1000
Loading : 25 μg

Anti-SOX9 antibody [GT1219] used in Immunoprecipitation (IP). GTX01545

GTX01545 IP Image

IP analysis of HeLa cell lysate using GTX01545 SOX9 antibody [GT1219].
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-SOX9 antibody [GT1219] used in IHC (Paraffin sections) (IHC-P). GTX01545

GTX01545 IHC-P Image

IHC-P analysis of rat testis tissue section using GTX01545 SOX9 antibody [GT1219].
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399