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SP1 antibody [GT1220]

Recombinant antibody KO/KD
Anti-SP1 antibody [GT1220] used in Western Blot (WB). GTX01528
Anti-SP1 antibody [GT1220] used in Western Blot (WB). GTX01528
Anti-SP1 antibody [GT1220] used in IHC (Paraffin sections) (IHC-P). GTX01528
Anti-SP1 antibody [GT1220] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01528
Anti-SP1 antibody [GT1220] used in IHC (Paraffin sections) (IHC-P). GTX01528
Anti-SP1 antibody [GT1220] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01528
Anti-SP1 antibody [GT1220] used in Western Blot (WB). GTX01528

Cat. No. GTX01528

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1220

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all SP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human SP1.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SP1,Sp1 transcription factor

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]

Database

Research Area

DATA IMAGES

Anti-SP1 antibody [GT1220] used in Western Blot (WB). GTX01528

GTX01528 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [GT1220] (GTX01528) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SP1 antibody [GT1220] used in Western Blot (WB). GTX01528

GTX01528 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [GT1220] (GTX01528) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 30463940

Anti-SP1 antibody [GT1220] used in IHC (Paraffin sections) (IHC-P). GTX01528

GTX01528 IHC-P Image

IHC-P analysis of rat spleen tissue section using GTX01528 SP1 antibody [GT1220].
Dilution : 1:100

Anti-SP1 antibody [GT1220] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01528

GTX01528 ICC/IF Image

ICC/IF analysis of C6 cells using GTX01528 SP1 antibody [GT1220].
Blue : DAPI

Anti-SP1 antibody [GT1220] used in IHC (Paraffin sections) (IHC-P). GTX01528

GTX01528 IHC-P Image

IHC-P analysis of mouse kidney tissue section using GTX01528 SP1 antibody [GT1220].
Dilution : 1:100

Anti-SP1 antibody [GT1220] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01528

GTX01528 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX01528 SP1 antibody [GT1220].
Blue : DAPI

Anti-SP1 antibody [GT1220] used in Western Blot (WB). GTX01528

GTX01528 WB Image

WB analysis of various samples using GTX01528 SP1 antibody [GT1220].
Dilution : 1:1000
Loading : 25 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399