SP1 antibody [GT2574]

Cat. No. GTX634352

Host	Mouse
Clonality	Monoclonal
Clone Name	GT2574
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

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Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SP1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888442

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Sp1 transcription factor

Cellular Localization

Nucleus

Background

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

Database

Gene ID: 6667 SP1
UniProt: P08047 SP1

Research Area

DATA IMAGES

GTX634352 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [GT2574] (GTX634352) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX634352 IHC-P Image

SP1 antibody [GT2574] detects SP1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
SP1 stained by SP1 antibody [GT2574] (GTX634352) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX634352 ICC/IF Image

SP1 antibody [GT2574] detects SP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SP1 stained by SP1 antibody [GT2574] (GTX634352) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.
Scale bar= 10μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty