SP1 antibody [GT2574] detects SP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SP1 stained by SP1 antibody [GT2574] (GTX634352) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.
Scale bar= 10μm.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [GT2574] (GTX634352) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

SP1 antibody [GT2574] detects SP1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
SP1 stained by SP1 antibody [GT2574] (GTX634352) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min