SP1 antibody [HL1396]

Cat. No. GTX636836
3
Cat. No.  GTX636836
Recombinant antibody KO/KD Overexpression Carrier-Free
Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836
GTX636836 WB Image
KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [HL1396] (GTX636836) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836
GTX636836 WB Image
Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [HL1396] (GTX636836) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836
GTX636836 IHC-P Image

SP1 antibody [HL1396] detects SP1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836
GTX636836 IHC-P Image

SP1 antibody [HL1396] detects SP1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissue.
SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Anti-SP1 antibody [HL1396] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636836
GTX636836 ICC/IF Image

SP1 antibody [HL1396] detects SP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

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Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836
GTX636836 IHC-P Image

SP1 antibody [HL1396] detects SP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836
GTX636836 IHC-P Image

SP1 antibody [HL1396] detects SP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

7 / 9
Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836
GTX636836 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [HL1396] (GTX636836) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

8 / 9
Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836
GTX636836 IHC-P Image

SP1 antibody [HL1396] detects SP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836
Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836
Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836
Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836
Anti-SP1 antibody [HL1396] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636836
Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836
Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836
Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836
Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836
This SP1 antibody [HL1396] (GTX636836) is a rabbit monoclonal antibody validated for detection of SP1 by WB, ICC/IF, and IHC-P. Reactivity has been shown in Human, Mouse, and Rat. Knockout/knockdown validation supports antibody specificity.
  • Host
    Rabbit
  • Clonality
    Monoclonal
  • Clone Name
    HL1396
  • Isotype
    IgG
  • Applications
    WB ICC/IF IHC-P
  • Reactivity
    Human, Mouse, Rat
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