APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SP1. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Sp1 transcription factor
Cellular Localization
Nucleus,Cytoplasm
Background
The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]
Database
Research Area
DATA IMAGES
![Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836](/templates/default/js/lightbox/images/loading.gif "Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836")
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GTX636836 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [HL1396] (GTX636836) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636836 IHC-P Image
SP1 antibody [HL1396] detects SP1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissue.SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636836 IHC-P Image
SP1 antibody [HL1396] detects SP1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissue.SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636836 ICC/IF Image
SP1 antibody [HL1396] detects SP1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
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GTX636836 IHC-P Image
SP1 antibody [HL1396] detects SP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636836 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [HL1396] (GTX636836) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636836 IHC-P Image
SP1 antibody [HL1396] detects SP1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636836 IHC-P Image
SP1 antibody [HL1396] detects SP1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for SP1 antibody [HL1396] (GTX636836). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SP1 antibody [HL1396] (GTX636836). Be the first to share your experience with this product.
![Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836](/upload/website/prouct_img/small/GTX636836/GTX636836_44748_20220819_WB_shRNA_watermark_22082402_182.webp "Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836")
![Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836](/upload/website/prouct_img/small/GTX636836/GTX636836_44748_20221228_IHC-P_multiple_R_22122901_959.webp "Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836")
![Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836](/upload/website/prouct_img/small/GTX636836/GTX636836_44748_20221228_IHC-P_multiple_M_22122901_188.webp "Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836")
![Anti-SP1 antibody [HL1396] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636836](/upload/website/prouct_img/small/GTX636836/GTX636836_44748_20221104_ICC_IF_22112219_116.webp "Anti-SP1 antibody [HL1396] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636836")
![Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836](/upload/website/prouct_img/small/GTX636836/GTX636836_44662_20220527_IHC-P_M_22062121_567.webp "Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836")
![Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836](/upload/website/prouct_img/small/GTX636836/GTX636836_44748_20220722_WB_22080923_641.webp "Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836")
![Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836](/upload/website/prouct_img/small/GTX636836/GTX636836_44748_20220805_IHC-P_1_22083119_503.webp "Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836")
![Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836](/upload/website/prouct_img/small/GTX636836/GTX636836_44748_20220805_IHC-P_22083119_299.webp "Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836")
![Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836](/upload/website/prouct_img/normal/GTX636836/GTX636836_44748_20220819_WB_shRNA_watermark_22082402_182.webp "Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836")
![Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836](/upload/website/prouct_img/normal/GTX636836/GTX636836_44748_20221228_IHC-P_multiple_R_22122901_959.webp "Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836")
![Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836](/upload/website/prouct_img/normal/GTX636836/GTX636836_44748_20221228_IHC-P_multiple_M_22122901_188.webp "Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836")
![Anti-SP1 antibody [HL1396] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636836](/upload/website/prouct_img/normal/GTX636836/GTX636836_44748_20221104_ICC_IF_22112219_116.webp "Anti-SP1 antibody [HL1396] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636836")
![Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836](/upload/website/prouct_img/normal/GTX636836/GTX636836_44662_20220527_IHC-P_M_22062121_567.webp "Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836")
![Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836](/upload/website/prouct_img/normal/GTX636836/GTX636836_44748_20220722_WB_22080923_641.webp "Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836")
![Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836](/upload/website/prouct_img/normal/GTX636836/GTX636836_44748_20220805_IHC-P_1_22083119_503.webp "Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836")
![Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836](/upload/website/prouct_img/normal/GTX636836/GTX636836_44748_20220805_IHC-P_22083119_299.webp "Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836")