*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
The peptide used to generate this antibody is available for purchase (GTX89511-PEP).
This antibody is expected to recognise both reported isoforms (NP_612482.2; NP_003100.1).
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
TBS pH7.3, 0.5% BSA
0.02% Sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Peptide with sequence CSRIESPNENSNNSQ, from the internal region of the protein sequence according to NP_612482.2; NP_003100.1.
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Sp1 transcription factor
Nucleus , Cytoplasm
The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]
GTX89511 WB Image
WB analysis of HeLa lysate using GTX89511 SP1 antibody, Internal.
Dilution : 0.3μg/ml
Loading : 30μg protein in RIPA buffer