APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.3-1μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89511-PEP).
Product Note
This antibody is expected to recognise both reported isoforms (NP_612482.2; NP_003100.1).
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence CSRIESPNENSNNSQ, from the internal region of the protein sequence according to NP_612482.2; NP_003100.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Sp1 transcription factor
Cellular Localization
Nucleus , Cytoplasm
Background
The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]
Database
Research Area
DATA IMAGES
|
GTX89511 WB Image
WB analysis of HeLa lysate using GTX89511 SP1 antibody, Internal. Dilution : 0.3μg/ml Loading : 30μg protein in RIPA buffer
|
REFERENCE
There are currently no references for SP1 antibody, Internal (GTX89511). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SP1 antibody, Internal (GTX89511). Be the first to share your experience with this product.