APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:1000-1:2000 |
| 1:100-1:500 |
| 1:50-1:200 |
Not tested in other applications.
Calculated MW
Observed MW
95~105 kDa. The observed M.W. is based on the following publication. PMID: 30463940
PROPERTIES
Form
Liquid
Buffer
1x TBS (pH7.4), 1% BSA, 40% Glycerol
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Sp1 transcription factor , SP1
Cellular Localization
Nucleus,Cytoplasm
Background
The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]
Database
Research Area
DATA IMAGES
![Anti-SP1 antibody [JF0950] used in Western Blot (WB). GTX01012](/templates/default/js/lightbox/images/loading.gif "Anti-SP1 antibody [JF0950] used in Western Blot (WB). GTX01012")
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GTX01012 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [JF0950] (GTX01012) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX01012 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [JF0950] (GTX01012) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 30463940
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GTX01012 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [JF0950] (GTX01012) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 30463940
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GTX01012 ICC/IF Image
ICC/IF analysis of PFA-fixed MCF-7 cells using GTX01012 SP1 antibody [JF0950].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS
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GTX01012 IHC-P Image
IHC-P analysis of human colon cancer tissue section using GTX01012 SP1 antibody [JF0950].
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GTX01012 IHC-P Image
IHC-P analysis of human breast carcinoma tissue section using GTX01012 SP1 antibody [JF0950].
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GTX01012 ICC/IF Image
ICC/IF analysis of PFA-fixed HeLa cells using GTX01012 SP1 antibody [JF0950].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS
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GTX01012 IHC-P Image
IHC-P analysis of human kidney tissue section using GTX01012 SP1 antibody [JF0950].
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GTX01012 ICC/IF Image
ICC/IF analysis of PFA-fixed SW480 cells using GTX01012 SP1 antibody [JF0950].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS
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REFERENCE
There are currently no references for SP1 antibody [JF0950] (GTX01012). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SP1 antibody [JF0950] (GTX01012). Be the first to share your experience with this product.
![Anti-SP1 antibody [JF0950] used in Western Blot (WB). GTX01012](/upload/website/prouct_img/small/GTX01012/GTX01012_HJ1121_20200306_WB_shRNA_watermark_20031101_133.jpg "Anti-SP1 antibody [JF0950] used in Western Blot (WB). GTX01012")
![Anti-SP1 antibody [JF0950] used in Western Blot (WB). GTX01012](/upload/website/prouct_img/small/GTX01012/GTX01012_HJ1121_20200214_WB_2_20021900_703.jpg "Anti-SP1 antibody [JF0950] used in Western Blot (WB). GTX01012")
![Anti-SP1 antibody [JF0950] used in Western Blot (WB). GTX01012](/upload/website/prouct_img/small/GTX01012/GTX01012_HJ1121_20200214_WB_20021900_870.jpg "Anti-SP1 antibody [JF0950] used in Western Blot (WB). GTX01012")
![Anti-SP1 antibody [JF0950] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01012](/upload/website/prouct_img/small/GTX01012/GTX01012_20200303_ICC-IF_171_20030421_136.jpg "Anti-SP1 antibody [JF0950] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01012")
![Anti-SP1 antibody [JF0950] used in IHC (Paraffin sections) (IHC-P). GTX01012](/upload/website/prouct_img/small/GTX01012/GTX01012_20200303_IHC-P_174_20030421_883.jpg "Anti-SP1 antibody [JF0950] used in IHC (Paraffin sections) (IHC-P). GTX01012")
![Anti-SP1 antibody [JF0950] used in IHC (Paraffin sections) (IHC-P). GTX01012](/upload/website/prouct_img/small/GTX01012/GTX01012_20200303_IHC-P_173_20030421_608.jpg "Anti-SP1 antibody [JF0950] used in IHC (Paraffin sections) (IHC-P). GTX01012")
![Anti-SP1 antibody [JF0950] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01012](/upload/website/prouct_img/small/GTX01012/GTX01012_20200303_ICC-IF_170_20030422_830.jpg "Anti-SP1 antibody [JF0950] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01012")
![Anti-SP1 antibody [JF0950] used in IHC (Paraffin sections) (IHC-P). GTX01012](/upload/website/prouct_img/small/GTX01012/GTX01012_20200303_IHC-P_175_20030422_976.jpg "Anti-SP1 antibody [JF0950] used in IHC (Paraffin sections) (IHC-P). GTX01012")
![Anti-SP1 antibody [JF0950] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01012](/upload/website/prouct_img/small/GTX01012/GTX01012_20200303_ICC-IF_172_20030422_593.jpg "Anti-SP1 antibody [JF0950] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01012")
![Anti-SP1 antibody [JF0950] used in Western Blot (WB). GTX01012](/upload/website/prouct_img/normal/GTX01012/GTX01012_HJ1121_20200306_WB_shRNA_watermark_20031101_133.jpg "Anti-SP1 antibody [JF0950] used in Western Blot (WB). GTX01012")
![Anti-SP1 antibody [JF0950] used in Western Blot (WB). GTX01012](/upload/website/prouct_img/normal/GTX01012/GTX01012_HJ1121_20200214_WB_2_20021900_703.jpg "Anti-SP1 antibody [JF0950] used in Western Blot (WB). GTX01012")
![Anti-SP1 antibody [JF0950] used in Western Blot (WB). GTX01012](/upload/website/prouct_img/normal/GTX01012/GTX01012_HJ1121_20200214_WB_20021900_870.jpg "Anti-SP1 antibody [JF0950] used in Western Blot (WB). GTX01012")
![Anti-SP1 antibody [JF0950] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01012](/upload/website/prouct_img/normal/GTX01012/GTX01012_20200303_ICC-IF_171_20030421_136.jpg "Anti-SP1 antibody [JF0950] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01012")
![Anti-SP1 antibody [JF0950] used in IHC (Paraffin sections) (IHC-P). GTX01012](/upload/website/prouct_img/normal/GTX01012/GTX01012_20200303_IHC-P_174_20030421_883.jpg "Anti-SP1 antibody [JF0950] used in IHC (Paraffin sections) (IHC-P). GTX01012")
![Anti-SP1 antibody [JF0950] used in IHC (Paraffin sections) (IHC-P). GTX01012](/upload/website/prouct_img/normal/GTX01012/GTX01012_20200303_IHC-P_173_20030421_608.jpg "Anti-SP1 antibody [JF0950] used in IHC (Paraffin sections) (IHC-P). GTX01012")
![Anti-SP1 antibody [JF0950] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01012](/upload/website/prouct_img/normal/GTX01012/GTX01012_20200303_ICC-IF_170_20030422_830.jpg "Anti-SP1 antibody [JF0950] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01012")
![Anti-SP1 antibody [JF0950] used in IHC (Paraffin sections) (IHC-P). GTX01012](/upload/website/prouct_img/normal/GTX01012/GTX01012_20200303_IHC-P_175_20030422_976.jpg "Anti-SP1 antibody [JF0950] used in IHC (Paraffin sections) (IHC-P). GTX01012")
![Anti-SP1 antibody [JF0950] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01012](/upload/website/prouct_img/normal/GTX01012/GTX01012_20200303_ICC-IF_172_20030422_593.jpg "Anti-SP1 antibody [JF0950] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01012")