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SP1 antibody [JF0950]

Recombinant antibody KO/KD
Anti-SP1 antibody [JF0950] used in Western Blot (WB). GTX01012
Anti-SP1 antibody [JF0950] used in Western Blot (WB). GTX01012
Anti-SP1 antibody [JF0950] used in Western Blot (WB). GTX01012
Anti-SP1 antibody [JF0950] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01012
Anti-SP1 antibody [JF0950] used in IHC (Paraffin sections) (IHC-P). GTX01012
Anti-SP1 antibody [JF0950] used in IHC (Paraffin sections) (IHC-P). GTX01012
Anti-SP1 antibody [JF0950] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01012
Anti-SP1 antibody [JF0950] used in IHC (Paraffin sections) (IHC-P). GTX01012
Anti-SP1 antibody [JF0950] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01012

Cat No. GTX01012

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 JF0950

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Observed MW

95~105 kDa. The observed M.W. is based on the following publication. PMID: 30463940

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Sp1 transcription factor,SP1

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]

Database

Research Area

DATA IMAGES

Anti-SP1 antibody [JF0950] used in Western Blot (WB). GTX01012

GTX01012 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [JF0950] (GTX01012) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SP1 antibody [JF0950] used in Western Blot (WB). GTX01012

GTX01012 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [JF0950] (GTX01012) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 30463940

Anti-SP1 antibody [JF0950] used in Western Blot (WB). GTX01012

GTX01012 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [JF0950] (GTX01012) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 30463940

Anti-SP1 antibody [JF0950] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01012

GTX01012 ICC/IF Image

ICC/IF analysis of PFA-fixed MCF-7 cells using GTX01012 SP1 antibody [JF0950].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-SP1 antibody [JF0950] used in IHC (Paraffin sections) (IHC-P). GTX01012

GTX01012 IHC-P Image

IHC-P analysis of human colon cancer tissue section using GTX01012 SP1 antibody [JF0950].

Anti-SP1 antibody [JF0950] used in IHC (Paraffin sections) (IHC-P). GTX01012

GTX01012 IHC-P Image

IHC-P analysis of human breast carcinoma tissue section using GTX01012 SP1 antibody [JF0950].

Anti-SP1 antibody [JF0950] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01012

GTX01012 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX01012 SP1 antibody [JF0950].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-SP1 antibody [JF0950] used in IHC (Paraffin sections) (IHC-P). GTX01012

GTX01012 IHC-P Image

IHC-P analysis of human kidney tissue section using GTX01012 SP1 antibody [JF0950].

Anti-SP1 antibody [JF0950] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01012

GTX01012 ICC/IF Image

ICC/IF analysis of PFA-fixed SW480 cells using GTX01012 SP1 antibody [JF0950].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

REFERENCE

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REVIEW

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Package List Price ($)
$ 369