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SP1 antibody

Anti-SP1 antibody used in Western Blot (WB). GTX103031
Anti-SP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103031
Anti-SP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103031
Anti-SP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103031
Anti-SP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103031
Anti-SP1 antibody used in Western Blot (WB). GTX103031

Cat No. GTX103031

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

THP-1

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176348

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Sp1 transcription factor

Cellular Localization

Nucleus

Background

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

Database

Research Area

DATA IMAGES

Anti-SP1 antibody used in Western Blot (WB). GTX103031

GTX103031 WB Image

SP1 antibody detects SP1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody (GTX103031) diluted by 1:500.

Anti-SP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103031

GTX103031 IHC-P Image

SP1 antibody detects SP1 protein at nucleus on human gastric carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human gastric carcinoma.
SP1 antibody (GTX103031) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103031

GTX103031 ICC/IF Image

SP1 antibody detects SP1 protein at nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SP1 protein stained by SP1 antibody (GTX103031) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 6 μm.

Anti-SP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103031

GTX103031 IHC-P Image

SP1 antibody detects SP1 protein at nucleus on human gastric carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human gastric carcinoma.
SP1 antibody (GTX103031) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103031

GTX103031 IHC-P Image

SP1 antibody detects SP1 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
SP1 antibody (GTX103031) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SP1 antibody used in Western Blot (WB). GTX103031

GTX103031 WB Image

Sample (30 ug of whole cell lysate)
A: THP-1
5% SDS PAGE
GTX103031 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169