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SP1 antibody

Anti-SP1 antibody used in Western Blot (WB). GTX110593
Anti-SP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110593
Anti-SP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110593
Anti-SP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110593
Anti-SP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110593
Anti-SP1 antibody used in Western Blot (WB). GTX110593
Anti-SP1 antibody used in ChIP assay (ChIP assay). GTX110593
Anti-SP1 antibody used in Immunoprecipitation (IP). GTX110593
Anti-SP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110593

Cat No. GTX110593

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

293T , K562 , THP-1 , HL-60 , *Raji

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176980

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Sp1 transcription factor

Cellular Localization

Nucleus

Background

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

Database

Research Area

DATA IMAGES

Anti-SP1 antibody used in Western Blot (WB). GTX110593

GTX110593 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody (GTX110593) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110593

GTX110593 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using SP1(GTX110593) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110593

GTX110593 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using SP1(GTX110593) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110593

GTX110593 ICC/IF Image

SP1 antibody detects SP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SP1 stained by SP1 antibody (GTX110593) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.

Anti-SP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110593

GTX110593 IHC-P Image

SP1 antibody detects SP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SP1 stained by SP1 antibody (GTX110593) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SP1 antibody used in Western Blot (WB). GTX110593

GTX110593 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody (GTX110593) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SP1 antibody used in ChIP assay (ChIP assay). GTX110593

GTX110593 ChIP assay Image

ChIP was performed with 293T chromatin extract and 5 μg of either normal rabbit IgG or anti-SP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to MGARP promoter.

Anti-SP1 antibody used in Immunoprecipitation (IP). GTX110593

GTX110593 IP Image

Immunoprecipitation of SP1 protein from THP-1 whole cell extracts using 5 μg of OCT1 antibody (GTX110593).
Western blot analysis was performed using OCT1 antibody (GTX110593) diluted at 1:250.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110593

GTX110593 IHC-P Image

SP1 antibody detects SP1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
SP1 stained by SP1 antibody (GTX110593) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

SP1 antibody Cat No. GTX110593

Rating ( Average 4.8 based on 5 users reviews)
Application
Western Blot(WB) ( Average 4.8 based on 4 users reviews)
ChIP assay(ChIP assay) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ChIP assay
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Pharmacol Res Perspect in 2018.PMID: 30455960
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cells in 2020.PMID: 32290085
Date : Anonymous submitted on 09-Aug-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : OVSAYO cells
Blocking : 3% skim milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Date : Anonymous submitted on 30-Mar-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : PC3 cells
Amount used : 20μg
Blocking : 5% milk, 4°C, 1Hr
Primary Antibody : 1:3000, 4°C, 12Hr
Date : Anonymous submitted on 09-Jan-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : Raji cells
Amount used : 50μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf