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SP1 (phospho Thr739) antibody

Anti-SP1 (phospho Thr739) antibody used in Western Blot (WB). GTX50710

Cat. No. GTX50710

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all SP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 739 (T-A-T(p)-P-S) derived from human SP1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11163554

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Sp1 transcription factor

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]

Database

Research Area

DATA IMAGES

Anti-SP1 (phospho Thr739) antibody used in Western Blot (WB). GTX50710

GTX50710 WB Image

WB analysis of extracts from 3T3 cells treated with starvation using GTX50710 SP1 (phospho Thr739) antibody.

REFERENCE

Application Reference

Wu W et al. Cell Mol Immunol 2018; (Epub) Cystathionine-γ-lyase ameliorates the histone demethylase JMJD3-mediated autoimmune response in rheumatoid arthritis.
Application : WB
Reactivity : Mouse
Wu YH et al. Int J Cancer 2017; (Epub) Activation of TWIST1 by COL11A1 promotes chemoresistance and inhibits apoptosis in ovarian cancer cells by modulating NF-κB-mediated IKKβ expression.
Application : WB
Reactivity : Human
Carrasco-Torres G et al. Oxid Med Cell Longev 2017; 2017 : 4674918 Quercetin reverses rat liver preneoplastic lesions induced by chemical carcinogenesis.
Application : WB
Reactivity : Rat
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399