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SP100 antibody

Anti-SP100 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131570
Anti-SP100 antibody used in Western Blot (WB). GTX131570

Cat No. GTX131570

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Observed MW

100~140 kDa. The observed M.W. is based on the following publication. PMID: 21172801

Positive Control

A431 , HeLa , HepG2
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SP100. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

SP100 nuclear antigen,lysp100b

Cellular Localization

Nucleus

Database

DATA IMAGES
Anti-SP100 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131570

GTX131570 ICC/IF Image

SP100 antibody detects SP100 protein at P-body and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SP100 protein stained by SP100 antibody (GTX131570) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-SP100 antibody used in Western Blot (WB). GTX131570

GTX131570 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SP100 antibody (GTX131570) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 21172801

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169