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SP2 antibody

Comparison
Anti-SP2 antibody used in Western Blot (WB). GTX129030
Anti-SP2 antibody used in Western Blot (WB). GTX129030
Anti-SP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129030
Anti-SP2 antibody used in Immunoprecipitation (IP). GTX129030
Anti-SP2 antibody used in Western Blot (WB). GTX129030
Anti-SP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129030

Cat. No. GTX129030

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

A431 , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885876

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Sp2 transcription factor

Cellular Localization

Nucleus

Background

This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein contains the least conserved DNA-binding domain within the Sp subfamily of proteins, and its DNA sequence specificity differs from the other Sp proteins. It localizes primarily within subnuclear foci associated with the nuclear matrix, and can activate or in some cases repress expression from different promoters. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SP2 antibody used in Western Blot (WB). GTX129030

GTX129030 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP2 antibody (GTX129030) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SP2 antibody used in Western Blot (WB). GTX129030

GTX129030 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP2 antibody (GTX129030) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129030

GTX129030 IHC-P Image

SP2 antibody detects SP2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
SP2 stained by SP2 antibody (GTX129030) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SP2 antibody used in Immunoprecipitation (IP). GTX129030

GTX129030 IP Image

SP2 antibody immunoprecipitates SP2 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : 30 μg whole cell lysate/extract of SP2 protein expressing Raji cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of SP2 by 3 μg of SP2 antibody (GTX129030) 5% SDS-PAGE The immunoprecipitated SP2 protein was detected by SP2 antibody (GTX129030) diluted at 1 : 500. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-SP2 antibody used in Western Blot (WB). GTX129030

GTX129030 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with SP2 antibody (GTX129030) diluted at 1:1000 and competitor's antibody (SC-643) diluted by 1:200.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-SP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129030

GTX129030 ICC/IF Image

SP2 antibody detects SP2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SP2 stained by SP2 antibody (GTX129030) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169