GeneTex
United States (US)

SPAG5 antibody

GTX115448_41227_IFA_18121410_401.jpg

Cat No. GTX115448

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SPAG5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

sperm associated antigen 5 Antibody, hMAP126 Antibody, DEEPEST Antibody, MAP126 Antibody

Cellular Localization

Cytoplasm , Spindle , Kinetochore

Background

This gene encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles. [provided by RefSeq]
Show More +

Database

Research Area

DATA IMAGES
GTX115448_41227_IFA_18121410_401.jpg

GTX115448 ICC/IF Image

SPAG5 antibody detects SPAG5 protein at spindle by immunofluorescent analysis.
Sample: HeLa cells were fixed in 100% MeOH for 5 min.
Green: SPAG5 protein stained by SPAG5 antibody (GTX115448) diluted at 1:500.
Blue: Hoechst 33342 staining.

SDS
Glycerol.pdf
Thimerosal.pdf