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SPAG5 antibody

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Anti-SPAG5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115449
Anti-SPAG5 antibody used in Western Blot (WB). GTX115449
Anti-SPAG5 antibody used in Western Blot (WB). GTX115449

Cat. No. GTX115449

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SPAG5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NT2D1

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SPAG5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885366

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sperm associated antigen 5 , DEEPEST , MAP126 , hMAP126

Cellular Localization

Cytoplasm , Spindle , Kinetochore

Background

This gene encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SPAG5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115449

GTX115449 ICC/IF Image

SPAG5 antibody detects SPAG5 protein at spindle by immunofluorescent analysis.
Sample: HeLa cells were fixed in 100% MeOH for 5 min.
Green: SPAG5 protein stained by SPAG5 antibody (GTX115449) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-SPAG5 antibody used in Western Blot (WB). GTX115449

GTX115449 WB Image

SPAG5 antibody detects SPAG5 protein by western blot analysis.
A. 30 μg NT2D1 whole cell extract
5 % SDS-PAGE
SPAG5 antibody (GTX115449) dilution: 1:500

Anti-SPAG5 antibody used in Western Blot (WB). GTX115449

GTX115449 WB Image

SPAG5 antibody detects SPAG5 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg A431 whole cell extract
C. 30 μg HeLa whole cell extract
D. 30 μg HepG2 whole cell extract
5 % SDS-PAGE
SPAG5 antibody (GTX115449) dilution: 1:500

REFERENCE

Application Reference

Ying Z et al. BMC Biol 2022; 20 (1) : 240 CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72.
Application : WB, ICC/IF
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169