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SPARC antibody, C-term

Anti-SPARC antibody, C-term used in Western Blot (WB). GTX81749
Anti-SPARC antibody, C-term used in Western Blot (WB). GTX81749
Anti-SPARC antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81749
Anti-SPARC antibody, C-term used in Flow cytometry (FACS). GTX81749

Cat. No. GTX81749

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all SPARC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:10-1:50
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Chicken, Pig, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 224-251 amino acids from the C-terminal region of human SPARC.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

secreted protein acidic and cysteine rich , BM-40 , OI17 , ON

Background

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]

Database

Research Area

DATA IMAGES

Anti-SPARC antibody, C-term used in Western Blot (WB). GTX81749

GTX81749 WB Image

WB analysis of Y79 cell lysate (35ug/lane) using GTX81749 SPARC antibody, C-term.

Anti-SPARC antibody, C-term used in Western Blot (WB). GTX81749

GTX81749 WB Image

WB analysis of 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPARC (Lane 2) using GTX81749 SPARC antibody, C-term.

Anti-SPARC antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81749

GTX81749 IHC-P Image

IHC-P analysis of human skin tissue using GTX81749 SPARC antibody, C-term.

Anti-SPARC antibody, C-term used in Flow cytometry (FACS). GTX81749

GTX81749 FACS Image

FACS analysis of WiDr cells using GTX81749 SPARC antibody, C-term.
Top histogram : negative control
Bottom histogram : WiDr cells

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399