Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with SPARC antibody (GTX133747) diluted at 1:1000 and competitor's antibody (sc-25574) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SPARC antibody detects SPARC protein in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
SPARC antibody (GTX133747) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SPARC antibody detects SPARC protein by immunohistochemical analysis.
Samples: Paraffin-embedded mouse retina.
Green: SPARC protein stained by SPARC antibody (GTX133747) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SPARC antibody detects SPARC protein in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Antibodies: SPARC antibody (GTX133747) diluted at 1:500, and competitor's antibody diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SPARC antibody (GTX133747) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SPARC antibody detects SPARC protein in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
SPARC antibody (GTX133747) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

The data was published in the 2022 in Clin Exp Dent Res. PMID: 35106969