GeneTex
United States (US)

SPARTIN antibody, Internal

GTX88225_2286_WB_18121411_547.jpg
GTX88225_2668_ICC-IF_18121411_783.jpg

Cat No. GTX88225

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88225-PEP).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-EASGTDVKQLDQGNK, from the internal region of the protein sequence according to NP_055902.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Spartin,Spg20,Tahccp1,Spart

Cellular Localization

Transiently associated with endosomes.

Background

This gene encodes a protein containing a MIT (Microtubule Interacting and Trafficking molecule) domain, and is implicated in regulating endosomal trafficking and mitochondria function. The protein localizes to mitochondria and partially co-localizes with microtubules. Stimulation with epidermal growth factor (EGF) results in protein translocation to the plasma membrane, and the protein functions in the degradation and intracellular trafficking of EGF receptor. Multiple alternatively spliced variants, encoding the same protein, have been identified. Mutations associated with this gene cause autosomal recessive spastic paraplegia 20 (Troyer syndrome). [provided by RefSeq, Nov 2008]

Database

Research Area

DATA IMAGES
GTX88225_2286_WB_18121411_547.jpg

GTX88225 WB Image

WB analysis of HeLa lysate using GTX88225 SPARTIN antibody, Internal.
Dilution : 2µg/ml
Loading : 35µg protein in RIPA buffer

GTX88225_2668_ICC-IF_18121411_783.jpg

GTX88225 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa before (left) and after (right) introduction of siRNA using GTX88225 SPARTIN antibody, Internal.
Dilution : 2µg/ml

Package List Price ($)
$ 329