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SPFH2 antibody, C-term

Anti-SPFH2 antibody, C-term used in Western Blot (WB). GTX89522
Anti-SPFH2 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89522

Cat. No. GTX89522

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all SPFH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89522-PEP).

Product Note

This antibody is expected to recognise isoform 1 (NP_009106) only.

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-EDEPLETATKEN, from the C Terminus of the protein sequence according to NP_009106.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ER lipid raft associated 2 , C8orf2 , Erlin-2 , NET32 , SPFH2 , SPG18

Cellular Localization

Endoplasmic reticulum membrane

Background

This gene encodes a member of the SPFH domain-containing family of lipid raft-associated proteins. The encoded protein is localized to lipid rafts of the endoplasmic reticulum and plays a critical role in inositol 1,4,5-trisphosphate (IP3) signaling by mediating ER-associated degradation of activated IP3 receptors. Mutations in this gene are a cause of spastic paraplegia-18 (SPG18). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES

Anti-SPFH2 antibody, C-term used in Western Blot (WB). GTX89522

GTX89522 WB Image

WB analysis of human breast lysate using GTX89522 SPFH2 antibody, C-term.
Dilution : 1μg/ml
Loading : 30μg protein in RIPA buffer

Anti-SPFH2 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89522

GTX89522 IHC-P Image

IHC-P analysis of human breast using GTX89522 SPFH2 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399