APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-1μg/ml |
| 3.75μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89522-PEP).
Product Note
This antibody is expected to recognise isoform 1 (NP_009106) only.
Predict Reactivity
Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-EDEPLETATKEN, from the C Terminus of the protein sequence according to NP_009106.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ER lipid raft associated 2 , C8orf2 , Erlin-2 , NET32 , SPFH2 , SPG18
Cellular Localization
Endoplasmic reticulum membrane
Background
This gene encodes a member of the SPFH domain-containing family of lipid raft-associated proteins. The encoded protein is localized to lipid rafts of the endoplasmic reticulum and plays a critical role in inositol 1,4,5-trisphosphate (IP3) signaling by mediating ER-associated degradation of activated IP3 receptors. Mutations in this gene are a cause of spastic paraplegia-18 (SPG18). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]
Database
Research Area
DATA IMAGES
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GTX89522 WB Image
WB analysis of human breast lysate using GTX89522 SPFH2 antibody, C-term.
Dilution : 1μg/ml
Loading : 30μg protein in RIPA buffer
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GTX89522 IHC-P Image
IHC-P analysis of human breast using GTX89522 SPFH2 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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REFERENCE
There are currently no references for SPFH2 antibody, C-term (GTX89522). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SPFH2 antibody, C-term (GTX89522). Be the first to share your experience with this product.
