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SPFH2 antibody [C2C3], C-term

Anti-SPFH2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106277
Anti-SPFH2 antibody [C2C3], C-term used in Western Blot (WB). GTX106277

Cat. No. GTX106277

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($419),
25 μl ($169)
See all SPFH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

HeLaS3

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SPFH2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950226

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ER lipid raft associated 2 , C8orf2 , Erlin-2 , NET32 , SPFH2 , SPG18

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type II membrane protein

Background

Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Also involved in ITPR1 degradation by the ERAD pathway.

Database

Research Area

DATA IMAGES

Anti-SPFH2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106277

GTX106277 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human endo mitral ovarian cancer, using ERLIN2(GTX106277) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SPFH2 antibody [C2C3], C-term used in Western Blot (WB). GTX106277

GTX106277 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
12% SDS PAGE
GTX106277 diluted at 1:500

REFERENCE

Application Reference

Tien SC et al. iScience 2024; 27 (7) : 110346 Exosomal miRNA 16-5p/29a-3p from pancreatic cancer induce adipose atrophy by inhibiting adipogenesis and promoting lipolysis.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169