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SPG7 antibody [HL2236]

Recombinant antibody Orthogonal
Anti-SPG7 antibody [HL2236] used in IHC (Paraffin sections) (IHC-P). GTX638281
Anti-SPG7 antibody [HL2236] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638281
Anti-SPG7 antibody [HL2236] used in Western Blot (WB). GTX638281
Anti-SPG7 antibody [HL2236] used in Western Blot (WB). GTX638281
Anti-SPG7 antibody [HL2236] used in Western Blot (WB). GTX638281

Cat. No. GTX638281

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2236

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SPG7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:20-1:1000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragmemt of human SPG7.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SPG7 matrix AAA peptidase subunit, paraplegin , CAR , CMAR , PGN , SPG5C

Cellular Localization

Mitochondrion inner membrane

Background

This gene encodes a mitochondrial metalloprotease protein that is a member of the AAA family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Mutations in this gene cause autosomal recessive spastic paraplegia 7. Two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES

Anti-SPG7 antibody [HL2236] used in IHC (Paraffin sections) (IHC-P). GTX638281

GTX638281 IHC-P Image

SPG7 antibody [HL2236] detects SPG7 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
SPG7 stained by SPG7 antibody [HL2236] (GTX638281) diluted at 1:20.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SPG7 antibody [HL2236] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638281

GTX638281 ICC/IF Image

SPG7 antibody [HL2236] detects SPG7 protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: SPG7 stained by SPG7 antibody [HL2236] (GTX638281) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SPG7 antibody [HL2236] used in Western Blot (WB). GTX638281

GTX638281 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (GTX638281) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SPG7 antibody [HL2236] used in Western Blot (WB). GTX638281

GTX638281 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (GTX638281) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-SPG7 antibody [HL2236] used in Western Blot (WB). GTX638281

GTX638281 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (GTX638281) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations