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SPHK1 antibody [C3], C-term

Anti-SPHK1 antibody [C3], C-term used in Western Blot (WB). GTX104724
Anti-SPHK1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104724
Anti-SPHK1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104724

Cat No. GTX104724

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

A431 , Molt-4
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SPHK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

sphingosine kinase 1 , SPHK

Background

Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-SPHK1 antibody [C3], C-term used in Western Blot (WB). GTX104724

GTX104724 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
10% SDS PAGE
GTX104724 diluted at 1:500

Anti-SPHK1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104724

GTX104724 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using SPHK1(GTX104724) antibody at 1:200 dilution.

Anti-SPHK1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104724

GTX104724 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using SPHK1(GTX104724) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
There are currently no references for SPHK1 antibody [C3], C-term (GTX104724). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SPHK1 antibody [C3], C-term (GTX104724). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169