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SPHK1 antibody [C3], C-term

KO/KD Orthogonal
Anti-SPHK1 antibody [C3], C-term used in Western Blot (WB). GTX104724
Anti-SPHK1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104724
Anti-SPHK1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104724
Anti-SPHK1 antibody [C3], C-term used in Western Blot (WB). GTX104724

Cat. No. GTX104724

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SPHK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

Molt-4

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SPHK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241356

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sphingosine kinase 1 , SPHK

Background

Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SPHK1 antibody [C3], C-term used in Western Blot (WB). GTX104724

GTX104724 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SPHK1 antibody [C3], C-term (GTX104724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SPHK1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104724

GTX104724 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using SPHK1(GTX104724) antibody at 1:200 dilution.

Anti-SPHK1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104724

GTX104724 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using SPHK1(GTX104724) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SPHK1 antibody [C3], C-term used in Western Blot (WB). GTX104724

GTX104724 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SPHK1 antibody [C3], C-term (GTX104724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169