Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SPHK1(GTX107509) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Sample (30μg whole cell lysate)
A:Hep G2 (GTX27900)
7.5% SDS PAGE
GTX107509 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using SPHK1(GTX107509) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min