GeneTex
United States (US)

SPHK1 antibody

GTX107843_40093_IFA_19011507_822.jpg
GTX107843_WB_19011507_298.jpg

Cat No. GTX107843

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SPHK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

SPHK antibody, SPHK1 antibody, sphingosine kinase 1 antibody, SPK 1 antibody, SK 1 antibody

Background

Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM]
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Database

Research Area

DATA IMAGES
GTX107843_40093_IFA_19011507_822.jpg

GTX107843 ICC/IF Image

SPHK1 antibody detects SPHK1 protein at nucleus and cytoplasm by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SPHK1 protein stained by SPHK1 antibody (GTX107843) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX107843_WB_19011507_298.jpg

GTX107843 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
10% SDS PAGE
GTX107843 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf