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SPHK1 antibody

Anti-SPHK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33516
Anti-SPHK1 antibody used in Western Blot (WB). GTX33516
Anti-SPHK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33516

Cat No. GTX33516

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 50-384 of human SPHK1 (NP_001136074.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

sphingosine kinase 1 , SPHK

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Phosphorylation of this protein alters its catalytic activity and promotes its translocation to the plasma membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2017]

Database

Research Area

DATA IMAGES

Anti-SPHK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33516

GTX33516 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX33516 SPHK1 antibody.
Dilution : 1:200

Anti-SPHK1 antibody used in Western Blot (WB). GTX33516

GTX33516 WB Image

WB analysis of various sample lysates using GTX33516 SPHK1 antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-SPHK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33516

GTX33516 IHC-P Image

IHC-P analysis of rat liver tissue using GTX33516 SPHK1 antibody.
Dilution : 1:100

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329