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SPHK1 antibody

Overexpression
Anti-SPHK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33516
Anti-SPHK1 antibody used in Western Blot (WB). GTX33516
Anti-SPHK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33516
Anti-SPHK1 antibody used in Western Blot (WB). GTX33516

Cat. No. GTX33516

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all SPHK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 50-384 of human SPHK1 (NP_001136074.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887717

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sphingosine kinase 1 , SPHK

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Phosphorylation of this protein alters its catalytic activity and promotes its translocation to the plasma membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2017]

Database

Research Area

DATA IMAGES

Anti-SPHK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33516

GTX33516 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX33516 SPHK1 antibody.
Dilution : 1:200

Anti-SPHK1 antibody used in Western Blot (WB). GTX33516

GTX33516 WB Image

WB analysis of various sample lysates using GTX33516 SPHK1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-SPHK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33516

GTX33516 IHC-P Image

IHC-P analysis of rat liver tissue using GTX33516 SPHK1 antibody.
Dilution : 1:100

Anti-SPHK1 antibody used in Western Blot (WB). GTX33516

GTX33516 WB Image

Overexpression

The data was published in the journal Aging (Albany NY) in 2019.PMID: 31809267

REFERENCE

Application Reference

Wang CQ et al. Aging (Albany NY) 2019; 11 (23) : 11040-11053 Sphingosine-1-phosphate promotes PDGF-dependent endothelial progenitor cell angiogenesis in human chondrosarcoma cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399