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SPOP antibody [N1C1]

Orthogonal Overexpression
Anti-SPOP antibody [N1C1] used in Western Blot (WB). GTX106942
Anti-SPOP antibody [N1C1] used in Western Blot (WB). GTX106942
Anti-SPOP antibody [N1C1] used in Western Blot (WB). GTX106942

Cat. No. GTX106942

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SPOP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

Mouse brain , SPOP-transfected 293T

Predict Reactivity

Rat, Zebrafish, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SPOP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951997

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

speckle type BTB/POZ protein , BTBD32 , TEF2

Cellular Localization

Nucleus

Background

This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SPOP antibody [N1C1] used in Western Blot (WB). GTX106942

GTX106942 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX106942 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SPOP antibody [N1C1] used in Western Blot (WB). GTX106942

GTX106942 WB Image

Orthogonal

HepG2 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SPOP antibody [N1C1] (GTX106942) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SPOP antibody [N1C1] used in Western Blot (WB). GTX106942

GTX106942 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SPOP antibody [N1C1] (GTX106942) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

He Y et al. Cancer Cell Int 2021; 21 (1) : 626 Clinicopathological and prognostic significance of speckle-type POZ protein in cancers: a systematic review and meta-analysis.
Luo J et al. Biochem Biophys Res Commun 2018; 504 (1) : 289-294 SPOP promotes FADD degradation and inhibits NF-κB activity in non-small cell lung cancer.
Application : WB
Reactivity : Human
Luo J et al. Biochem Biophys Res Commun 2017; (Epub) SPOP promotes SIRT2 degradation and suppresses non-small cell lung cancer cell growth.
Kim MS et al. APMIS 2013; 121 (7) : 626-33 Mutational and expressional analyses of SPOP, a candidate tumor suppressor gene, in prostate, gastric and colorectal cancers.
Application : IHC
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169