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SPRED2 antibody

Anti-SPRED2 antibody used in Western Blot (WB). GTX117851
Anti-SPRED2 antibody used in IHC (Paraffin sections) (IHC-P). GTX117851

Cat. No. GTX117851

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SPRED2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SPRED2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165000

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sprouty related EVH1 domain containing 2 , Spred-2

Cellular Localization

Membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasmic vesicle , secretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential) , Cytoplasm

Background

SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SPRED2 antibody used in Western Blot (WB). GTX117851

GTX117851 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX117851 diluted at 1:1000

Anti-SPRED2 antibody used in IHC (Paraffin sections) (IHC-P). GTX117851

GTX117851 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using SPRED2(GTX117851) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Cho CK et al. J Proteome Res 2010; 9 (7) : 3574-82 Amniotic fluid proteome analysis from Down syndrome pregnancies for biomarker discovery.
Application : ELISA
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169