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SPRY4 antibody

Anti-SPRY4 antibody used in Western Blot (WB). GTX119230
Anti-SPRY4 antibody used in IHC (Paraffin sections) (IHC-P). GTX119230
Anti-SPRY4 antibody used in Western Blot (WB). GTX119230
Anti-SPRY4 antibody used in IHC (Paraffin sections) (IHC-P). GTX119230

Cat. No. GTX119230

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SPRY4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

Mouse brain , NT2D1

Predict Reactivity

Rat, Sheep, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SPRY4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617727

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sprouty RTK signaling antagonist 4 , HH17

Cellular Localization

Cytoplasm , Cell projection , ruffle membrane; Peripheral membrane protein; Cytoplasmic side

Background

SPRY4 is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. It is positioned upstream of RAS (see HRAS; MIM 190020) activation and impairs the formation of active GTP-RAS (Leeksma et al., 2002 [PubMed 12027893]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SPRY4 antibody used in Western Blot (WB). GTX119230

GTX119230 WB Image

SPRY4 antibody detects SPRY4 protein by Western blot analysis.
A. 30 μg NT2D1 whole cell lysate/extract
12 % SDS-PAGE
SPRY4 antibody (GTX119230) dilution: 1:500

Anti-SPRY4 antibody used in IHC (Paraffin sections) (IHC-P). GTX119230

GTX119230 IHC-P Image

SPRY4 antibody detects SPRY4 protein at cytoplasm on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
SPRY4 antibody (GTX119230) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SPRY4 antibody used in Western Blot (WB). GTX119230

GTX119230 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX119230 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SPRY4 antibody used in IHC (Paraffin sections) (IHC-P). GTX119230

GTX119230 IHC-P Image

SPRY4 antibody detects SPRY4 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
SPRY4 antibody (GTX119230) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Kozar I. et al. Cancers 2021; 13 (5) : 1096 Cross-Linking Ligation and Sequencing of Hybrids (qCLASH) Reveals an Unpredicted miRNA Targetome in Melanoma Cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169