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SPRY4 antibody

Anti-SPRY4 antibody used in IHC (Paraffin sections) (IHC-P). GTX20265
Anti-SPRY4 antibody used in IHC (Paraffin sections) (IHC-P). GTX20265
Anti-SPRY4 antibody used in Western Blot (WB). GTX20265
Anti-SPRY4 antibody used in IHC (Paraffin sections) (IHC-P). GTX20265
Anti-SPRY4 antibody used in IHC (Paraffin sections) (IHC-P). GTX20265
Anti-SPRY4 antibody used in Western Blot (WB). GTX20265

Cat. No. GTX20265

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($589)
See all SPRY4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:1000
IHC-P 1-10 μg/mL
ELISA 1:3000-1:12000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to a C-Terminal region near amino acids 275-299 of Human Sprouty-4 protein.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sprouty RTK signaling antagonist 4 , HH17

Cellular Localization

Cytoplasm

Background

Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway downstream of FGF and EGF receptor tyrosine kinase activation. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS. SPRY4 is widely expressed, with different isoforms. The protein consists of 322 amino acids, with a cysteine-rich region, Src homology 3 binding regions, proline-rich regions, and a PEST sequence. It is expressed predominantly in the cytoplasm. Northern results show bands across most tissues, with strongest expression in heart, brain, placenta, lung, and intestine.

Database

Research Area

DATA IMAGES

Anti-SPRY4 antibody used in IHC (Paraffin sections) (IHC-P). GTX20265

GTX20265 IHC-P Image

Immunohistochemistry of Rabbit anti-Sprouty-4 antibody. Tissue: human heart tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: Sprouty-4 antibody at 2.5 μg/ml for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: Sprouty-4 is cytoplasmic.Staining: Sprouty-4 as precipitated red signal with hematoxylin purple nuclear counterstain.

Anti-SPRY4 antibody used in IHC (Paraffin sections) (IHC-P). GTX20265

GTX20265 IHC-P Image

Immunohistochemistry of Rabbit anti-Sprouty-4 antibody. Tissue: human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: Sprouty-4 antibody at 2.5 μg/mL for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: Sprouty-4 is cytoplasmic.Staining: Sprouty-4 as precipitated brown with blue nuclear counterstain.

Anti-SPRY4 antibody used in Western Blot (WB). GTX20265

GTX20265 WB Image

Western Blot of Rabbit anti-Sprouty-4 antibody. Lane 1: HeLa. Lane 2: SW13. Lane 3: C2C12. Load: 30 μg per lane.Primary antibody: Sprouty 4 antibody at 1:100 for overnight at 4ºC.Secondary antibody: IRDye800™ rabbit secondary antibody at 1:5,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4ºC.Predicted/Observed size: a doublet band ~35 kDa corresponding to human Sprouty-4. Other band(s): none.

Anti-SPRY4 antibody used in IHC (Paraffin sections) (IHC-P). GTX20265

GTX20265 IHC-P Image

IHC-P analysis of human liver tissue using GTX20265 SPRY4 antibody.
Dilution : 2.5 μg/mL

Anti-SPRY4 antibody used in IHC (Paraffin sections) (IHC-P). GTX20265

GTX20265 IHC-P Image

IHC-P analysis of human heart tissue using GTX20265 SPRY4 antibody.
Dilution : 2.5 μg/mL

Anti-SPRY4 antibody used in Western Blot (WB). GTX20265

GTX20265 WB Image

WB analysis of various samples using GTX20265 SPRY4 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : SW13 whole cell lysate
Lane 3 : C2C12 whole cell lysate
Loading : 30 μg
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 589