Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SPTBN1 antibody (GTX130935) diluted at 1:1000.

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SPTBN1 antibody (GTX130935) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SPTBN1 antibody detects SPTBN1 protein at cell membrane and cytoplasm in rat pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
SPTBN1 antibody (GTX130935) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min