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SPTBN2 antibody [SPTBN2/1247]

Anti-SPTBN2 antibody [SPTBN2/1247] used in IHC (Paraffin sections) (IHC-P). GTX35088
Anti-SPTBN2 antibody [SPTBN2/1247] used in IHC (Paraffin sections) (IHC-P). GTX35088
Anti-SPTBN2 antibody [SPTBN2/1247] used in IHC (Paraffin sections) (IHC-P). GTX35088

Cat No. GTX35088

Host Mouse
Clonality Monoclonal
Clone Name SPTBN2/1247
Isotype IgG3
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1.0ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

271 kDa. ( Note )

Specificity/Sensitivity

Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin α and spectrin β are present in erythrocytes, whereas spectrin α II (also designated fodrin α) and spectrin β I (also designated fodrin β) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin β III is highly homologous to both spectrin β I and spectrin β II. Spectrin β III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human SPTBN2 fragment (aa356-475) (exact sequence is proprietary)

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Spectrin Beta, Non-Erythrocytic 2,Gtrap41,Sca5,Scar14,Sptbn2

Background

Spectrins are principle components of a cell's membrane-cytoskeleton and are composed of two alpha and two beta spectrin subunits. The protein encoded by this gene (SPTBN2), is called spectrin beta non-erythrocytic 2 or beta-III spectrin. It is related to, but distinct from, the beta-II spectrin gene which is also known as spectrin beta non-erythrocytic 1 (SPTBN1). SPTBN2 regulates the glutamate signaling pathway by stabilizing the glutamate transporter EAAT4 at the surface of the plasma membrane. Mutations in this gene cause a form of spinocerebellar ataxia, SCA5, that is characterized by neurodegeneration, progressive locomotor incoordination, dysarthria, and uncoordinated eye movements. [provided by RefSeq, Dec 2009]

Database

Research Area

DATA IMAGES
Anti-SPTBN2 antibody [SPTBN2/1247] used in IHC (Paraffin sections) (IHC-P). GTX35088

GTX35088 IHC-P Image

Formalin-fixed, paraffin-embedded Human Pancreas stained with Spectrin beta III Monoclonal Antibody (SPTBN2/1247).

Anti-SPTBN2 antibody [SPTBN2/1247] used in IHC (Paraffin sections) (IHC-P). GTX35088

GTX35088 IHC-P Image

Formalin-fixed, paraffin-embedded Mouse Kidney stained with Spectrin beta III Monoclonal Antibody (SPTBN2/1247).

Anti-SPTBN2 antibody [SPTBN2/1247] used in IHC (Paraffin sections) (IHC-P). GTX35088

GTX35088 IHC-P Image

Formalin-fixed, paraffin-embedded Rat Pancreas stained with Spectrin beta III Monoclonal Antibody (SPTBN2/1247).

Package List Price ($)
$ 329