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SPTBN2 antibody [SPTBN2/1582]

Anti-SPTBN2 antibody [SPTBN2/1582] used in Protein Array (Protein Array). GTX35089
Anti-SPTBN2 antibody [SPTBN2/1582] used in IHC (Paraffin sections) (IHC-P). GTX35089
Anti-SPTBN2 antibody [SPTBN2/1582] used in Western Blot (WB). GTX35089
Anti-SPTBN2 antibody [SPTBN2/1582] used in Flow cytometry (FACS). GTX35089
Anti-SPTBN2 antibody [SPTBN2/1582] used in IHC (Paraffin sections) (IHC-P). GTX35089
Anti-SPTBN2 antibody [SPTBN2/1582] used in Western Blot (WB). GTX35089

Cat. No. GTX35089

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPTBN2/1582

Isotype

 IgG2a

Application

 WB, IHC-P, FACS, ELISA, Protein Array

Reactivity

 Human
Package
100 μg ($529)
See all SPTBN2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells
ELISA 2-4μg/ml (for coating)
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
Not tested in other applications.

Calculated MW

271 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa356-475) of human SPTBN2 protein (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GTRAP41 , SCA5 , SCAR14 , SPTBN2 , spectrin beta, nonerythrocytic 2 , Spectrin Beta III , spectrin β III , spectrin beta, non-erythrocytic 2

Cellular Localization

Cytoplasm,Cytoskeleton,Cell cortex

Background

Spectrins are principle components of a cell's membrane-cytoskeleton and are composed of two alpha and two beta spectrin subunits. The protein encoded by this gene (SPTBN2), is called spectrin beta non-erythrocytic 2 or beta-III spectrin. It is related to, but distinct from, the beta-II spectrin gene which is also known as spectrin beta non-erythrocytic 1 (SPTBN1). SPTBN2 regulates the glutamate signaling pathway by stabilizing the glutamate transporter EAAT4 at the surface of the plasma membrane. Mutations in this gene cause a form of spinocerebellar ataxia, SCA5, that is characterized by neurodegeneration, progressive locomotor incoordination, dysarthria, and uncoordinated eye movements. [provided by RefSeq, Dec 2009]

Database

Research Area

DATA IMAGES

Anti-SPTBN2 antibody [SPTBN2/1582] used in Protein Array (Protein Array). GTX35089

GTX35089 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using Spectrin beta III Mouse Monoclonal Antibody (SPTBN2/1582). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Anti-SPTBN2 antibody [SPTBN2/1582] used in IHC (Paraffin sections) (IHC-P). GTX35089

GTX35089 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX35089 SPTBN2 antibody [SPTBN2/1582].

Anti-SPTBN2 antibody [SPTBN2/1582] used in Western Blot (WB). GTX35089

GTX35089 WB Image

WB analysis of HeLa and 293 cell lysates using GTX35089 SPTBN2 antibody [SPTBN2/1582].

Anti-SPTBN2 antibody [SPTBN2/1582] used in Flow cytometry (FACS). GTX35089

GTX35089 FACS Image

FACS analysis of HeLa cells using GTX35089 SPTBN2 antibody [SPTBN2/1582].
Blue : Primary antibody
Red : Isotype control

Anti-SPTBN2 antibody [SPTBN2/1582] used in IHC (Paraffin sections) (IHC-P). GTX35089

GTX35089 IHC-P Image

IHC-P analysis of human histiocytoma tissue using GTX35089 Factor XIIIa antibody [F13A1/1448].

Anti-SPTBN2 antibody [SPTBN2/1582] used in Western Blot (WB). GTX35089

GTX35089 WB Image

WB analysis of HeLa cell lysate using GTX35089 SPTBN2 antibody [SPTBN2/1582].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529